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MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway.

Yang TQ, Lu XJ, Wu TF, Ding DD, Zhao ZH, Chen GL, Xie XS, Li B, Wei YX, Guo LC, Zhang Y, Huang YL, Zhou YX, Du ZW - Cancer Sci. (2014)

Bottom Line: MicroRNA-16 decreased glioma malignancy by downregulating NF-κB1 and MMP9, and led to suppressed invasiveness of human glioma cell lines SHG44, U87, and U373.Our results also indicated that upregulation of miR-16 promoted apoptosis by suppressing BCL2 expression.Finally, the upregulation of miR-16 in a nude mice model of human glioma resulted in significant suppression of glioma growth and invasiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.

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miR-16 reduces invasiveness of glioma cells. (a,b) Transwell invasion system showed that the numbers of invasive cells were significantly reduced compared with the cultures transfected with the negative control oligonucleotide. Each bar represents mean values ± SD from three independent experiments. **P < 0.01. (c) Expression of MMP9 was clearly inhibited by upregulation of microRNA-16 (miR-16).
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fig04: miR-16 reduces invasiveness of glioma cells. (a,b) Transwell invasion system showed that the numbers of invasive cells were significantly reduced compared with the cultures transfected with the negative control oligonucleotide. Each bar represents mean values ± SD from three independent experiments. **P < 0.01. (c) Expression of MMP9 was clearly inhibited by upregulation of microRNA-16 (miR-16).

Mentions: To assess the effects of miR-16 on the invasiveness of glioma cells, we used a Transwell invasion system. The numbers of invasive cells with miR-16 mimics were significantly reduced compared with those with the negative control oligonucleotide and mock (Fig.4a,b).


MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway.

Yang TQ, Lu XJ, Wu TF, Ding DD, Zhao ZH, Chen GL, Xie XS, Li B, Wei YX, Guo LC, Zhang Y, Huang YL, Zhou YX, Du ZW - Cancer Sci. (2014)

miR-16 reduces invasiveness of glioma cells. (a,b) Transwell invasion system showed that the numbers of invasive cells were significantly reduced compared with the cultures transfected with the negative control oligonucleotide. Each bar represents mean values ± SD from three independent experiments. **P < 0.01. (c) Expression of MMP9 was clearly inhibited by upregulation of microRNA-16 (miR-16).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317940&req=5

fig04: miR-16 reduces invasiveness of glioma cells. (a,b) Transwell invasion system showed that the numbers of invasive cells were significantly reduced compared with the cultures transfected with the negative control oligonucleotide. Each bar represents mean values ± SD from three independent experiments. **P < 0.01. (c) Expression of MMP9 was clearly inhibited by upregulation of microRNA-16 (miR-16).
Mentions: To assess the effects of miR-16 on the invasiveness of glioma cells, we used a Transwell invasion system. The numbers of invasive cells with miR-16 mimics were significantly reduced compared with those with the negative control oligonucleotide and mock (Fig.4a,b).

Bottom Line: MicroRNA-16 decreased glioma malignancy by downregulating NF-κB1 and MMP9, and led to suppressed invasiveness of human glioma cell lines SHG44, U87, and U373.Our results also indicated that upregulation of miR-16 promoted apoptosis by suppressing BCL2 expression.Finally, the upregulation of miR-16 in a nude mice model of human glioma resulted in significant suppression of glioma growth and invasiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.

Show MeSH
Related in: MedlinePlus