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MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway.

Yang TQ, Lu XJ, Wu TF, Ding DD, Zhao ZH, Chen GL, Xie XS, Li B, Wei YX, Guo LC, Zhang Y, Huang YL, Zhou YX, Du ZW - Cancer Sci. (2014)

Bottom Line: MicroRNA-16 decreased glioma malignancy by downregulating NF-κB1 and MMP9, and led to suppressed invasiveness of human glioma cell lines SHG44, U87, and U373.Our results also indicated that upregulation of miR-16 promoted apoptosis by suppressing BCL2 expression.Finally, the upregulation of miR-16 in a nude mice model of human glioma resulted in significant suppression of glioma growth and invasiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.

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MicroRNA-16 (miR-16) expression is inversely correlated with nuclear factor-κB1 (NF-κB1) in the same human brain glioma tissues. (a,c) MicroRNA-16 expression in human brain glioma tissues and human glioma cell lines was lower than in non-cancerous brain tissues (P < 0.01); miR-16 expression decreased with the increasing degree of malignancy. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. (b) Expression of NF-κB1 in human brain glioma tissues was higher than in non-cancerous brain tissues (P < 0.01). Expression of NF-κB1 correlated with the tumor grade. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. AA, anaplastic astrocytoma; DA, diffuse astrocytoma; GBM, primary brain glioblastoma; Norm, non-cancerous brain tissues; PA, pilocytic astrocytoma.
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fig01: MicroRNA-16 (miR-16) expression is inversely correlated with nuclear factor-κB1 (NF-κB1) in the same human brain glioma tissues. (a,c) MicroRNA-16 expression in human brain glioma tissues and human glioma cell lines was lower than in non-cancerous brain tissues (P < 0.01); miR-16 expression decreased with the increasing degree of malignancy. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. (b) Expression of NF-κB1 in human brain glioma tissues was higher than in non-cancerous brain tissues (P < 0.01). Expression of NF-κB1 correlated with the tumor grade. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. AA, anaplastic astrocytoma; DA, diffuse astrocytoma; GBM, primary brain glioblastoma; Norm, non-cancerous brain tissues; PA, pilocytic astrocytoma.

Mentions: To assess miR-16 and NF-κB1 expression in non-cancerous brain tissues and in different grades of glioma, we detected miR-16 expression and mRNA levels of NF-κB1 in six non-neoplastic brain tissues and in 29 human glioma tissue samples. Data showed that miR-16 expression in non-cancerous brain tissues was lower than in human brain glioma tissues (P < 0.01) and decreased with the increasing degree of malignancy in glioma (low-grade gliomas vs. high-grade gliomas, P < 0.05; Fig.1a). In addition, we found that the expression of miR-16 in glioma cell lines SHG44, U87, and U373 was lower than in non-cancerous brain tissues (P < 0.01; Fig.1c). However, the expression of NF-κB1 increased with the increasing degree of malignancy in glioma (low-grade gliomas vs. high-grade gliomas, P < 0.01; Fig.1b). Therefore, the expression of miR-16 and NF-κB1 was negatively correlated in glioma.


MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway.

Yang TQ, Lu XJ, Wu TF, Ding DD, Zhao ZH, Chen GL, Xie XS, Li B, Wei YX, Guo LC, Zhang Y, Huang YL, Zhou YX, Du ZW - Cancer Sci. (2014)

MicroRNA-16 (miR-16) expression is inversely correlated with nuclear factor-κB1 (NF-κB1) in the same human brain glioma tissues. (a,c) MicroRNA-16 expression in human brain glioma tissues and human glioma cell lines was lower than in non-cancerous brain tissues (P < 0.01); miR-16 expression decreased with the increasing degree of malignancy. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. (b) Expression of NF-κB1 in human brain glioma tissues was higher than in non-cancerous brain tissues (P < 0.01). Expression of NF-κB1 correlated with the tumor grade. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. AA, anaplastic astrocytoma; DA, diffuse astrocytoma; GBM, primary brain glioblastoma; Norm, non-cancerous brain tissues; PA, pilocytic astrocytoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317940&req=5

fig01: MicroRNA-16 (miR-16) expression is inversely correlated with nuclear factor-κB1 (NF-κB1) in the same human brain glioma tissues. (a,c) MicroRNA-16 expression in human brain glioma tissues and human glioma cell lines was lower than in non-cancerous brain tissues (P < 0.01); miR-16 expression decreased with the increasing degree of malignancy. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. (b) Expression of NF-κB1 in human brain glioma tissues was higher than in non-cancerous brain tissues (P < 0.01). Expression of NF-κB1 correlated with the tumor grade. Low-grade gliomas (grade I, II) versus high-grade gliomas (grade III, IV), P < 0.05. AA, anaplastic astrocytoma; DA, diffuse astrocytoma; GBM, primary brain glioblastoma; Norm, non-cancerous brain tissues; PA, pilocytic astrocytoma.
Mentions: To assess miR-16 and NF-κB1 expression in non-cancerous brain tissues and in different grades of glioma, we detected miR-16 expression and mRNA levels of NF-κB1 in six non-neoplastic brain tissues and in 29 human glioma tissue samples. Data showed that miR-16 expression in non-cancerous brain tissues was lower than in human brain glioma tissues (P < 0.01) and decreased with the increasing degree of malignancy in glioma (low-grade gliomas vs. high-grade gliomas, P < 0.05; Fig.1a). In addition, we found that the expression of miR-16 in glioma cell lines SHG44, U87, and U373 was lower than in non-cancerous brain tissues (P < 0.01; Fig.1c). However, the expression of NF-κB1 increased with the increasing degree of malignancy in glioma (low-grade gliomas vs. high-grade gliomas, P < 0.01; Fig.1b). Therefore, the expression of miR-16 and NF-κB1 was negatively correlated in glioma.

Bottom Line: MicroRNA-16 decreased glioma malignancy by downregulating NF-κB1 and MMP9, and led to suppressed invasiveness of human glioma cell lines SHG44, U87, and U373.Our results also indicated that upregulation of miR-16 promoted apoptosis by suppressing BCL2 expression.Finally, the upregulation of miR-16 in a nude mice model of human glioma resulted in significant suppression of glioma growth and invasiveness.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.

Show MeSH
Related in: MedlinePlus