MicroRNA-16 inhibits glioma cell growth and invasion through suppression of BCL2 and the nuclear factor-κB1/MMP9 signaling pathway.
Bottom Line: MicroRNA-16 decreased glioma malignancy by downregulating NF-κB1 and MMP9, and led to suppressed invasiveness of human glioma cell lines SHG44, U87, and U373.Our results also indicated that upregulation of miR-16 promoted apoptosis by suppressing BCL2 expression.Finally, the upregulation of miR-16 in a nude mice model of human glioma resulted in significant suppression of glioma growth and invasiveness.
Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.Show MeSH
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Mentions: To assess miR-16 and NF-κB1 expression in non-cancerous brain tissues and in different grades of glioma, we detected miR-16 expression and mRNA levels of NF-κB1 in six non-neoplastic brain tissues and in 29 human glioma tissue samples. Data showed that miR-16 expression in non-cancerous brain tissues was lower than in human brain glioma tissues (P < 0.01) and decreased with the increasing degree of malignancy in glioma (low-grade gliomas vs. high-grade gliomas, P < 0.05; Fig.1a). In addition, we found that the expression of miR-16 in glioma cell lines SHG44, U87, and U373 was lower than in non-cancerous brain tissues (P < 0.01; Fig.1c). However, the expression of NF-κB1 increased with the increasing degree of malignancy in glioma (low-grade gliomas vs. high-grade gliomas, P < 0.01; Fig.1b). Therefore, the expression of miR-16 and NF-κB1 was negatively correlated in glioma.
Affiliation: Department of Neurosurgery and Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, China.