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TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells.

Vo Nguyen TT, Watanabe Y, Shiba A, Noguchi M, Itoh S, Kato M - Cancer Sci. (2014)

Bottom Line: TMEPAI is constitutively and highly expressed in many types of cancer and is associated with poor prognosis.Knockdown of TMEPAI in Calu3 and NCI-H23 cells enhanced levels of Smad2 phosphorylation and significantly suppressed cell proliferation in the presence of TGF-β, indicating that highly expressed TMEPAI suppresses levels of Smad phosphorylation in these cancer cells and reduces the growth inhibitory effects of TGF-β/Smad signaling.Together, these experiments indicate that TMEPAI promotes tumorigenic activities in lung cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

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Related in: MedlinePlus

Calu3-shTMEPAI cells have decreased sphere-forming activities. (a) Control and TMEPAI-knockdown Calu3 cells (Calu3-sh#9 and Calu3-sh#10) were cultured in sphere formation medium for 12 days. Typical photographs are shown. (b) The numbers of spheres larger than 100 μm in diameter were counted. The means ± SDs are shown. *P < 0.01 (vs control). Calu3 sh. control, Calu3 sh. control expresses non-targeting shRNA (SHC002, Sigma) in pLKO.1-puro vector.
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fig04: Calu3-shTMEPAI cells have decreased sphere-forming activities. (a) Control and TMEPAI-knockdown Calu3 cells (Calu3-sh#9 and Calu3-sh#10) were cultured in sphere formation medium for 12 days. Typical photographs are shown. (b) The numbers of spheres larger than 100 μm in diameter were counted. The means ± SDs are shown. *P < 0.01 (vs control). Calu3 sh. control, Calu3 sh. control expresses non-targeting shRNA (SHC002, Sigma) in pLKO.1-puro vector.

Mentions: We further investigated the functional significance of TMEPAI in sphere-forming activities. The sphere-forming activities of TMEPAI knockdown cells were significantly reduced both in Calu3 (Fig.4a,b) and in NCI-H23 cells (Fig. S3d,e).


TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells.

Vo Nguyen TT, Watanabe Y, Shiba A, Noguchi M, Itoh S, Kato M - Cancer Sci. (2014)

Calu3-shTMEPAI cells have decreased sphere-forming activities. (a) Control and TMEPAI-knockdown Calu3 cells (Calu3-sh#9 and Calu3-sh#10) were cultured in sphere formation medium for 12 days. Typical photographs are shown. (b) The numbers of spheres larger than 100 μm in diameter were counted. The means ± SDs are shown. *P < 0.01 (vs control). Calu3 sh. control, Calu3 sh. control expresses non-targeting shRNA (SHC002, Sigma) in pLKO.1-puro vector.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317935&req=5

fig04: Calu3-shTMEPAI cells have decreased sphere-forming activities. (a) Control and TMEPAI-knockdown Calu3 cells (Calu3-sh#9 and Calu3-sh#10) were cultured in sphere formation medium for 12 days. Typical photographs are shown. (b) The numbers of spheres larger than 100 μm in diameter were counted. The means ± SDs are shown. *P < 0.01 (vs control). Calu3 sh. control, Calu3 sh. control expresses non-targeting shRNA (SHC002, Sigma) in pLKO.1-puro vector.
Mentions: We further investigated the functional significance of TMEPAI in sphere-forming activities. The sphere-forming activities of TMEPAI knockdown cells were significantly reduced both in Calu3 (Fig.4a,b) and in NCI-H23 cells (Fig. S3d,e).

Bottom Line: TMEPAI is constitutively and highly expressed in many types of cancer and is associated with poor prognosis.Knockdown of TMEPAI in Calu3 and NCI-H23 cells enhanced levels of Smad2 phosphorylation and significantly suppressed cell proliferation in the presence of TGF-β, indicating that highly expressed TMEPAI suppresses levels of Smad phosphorylation in these cancer cells and reduces the growth inhibitory effects of TGF-β/Smad signaling.Together, these experiments indicate that TMEPAI promotes tumorigenic activities in lung cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

Show MeSH
Related in: MedlinePlus