TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells.
Bottom Line: These results suggest that constitutive expression of TMEPAI in these cancer cells depends on autocrine TGF-β stimulation.Knockdown of TMEPAI in Calu3 and NCI-H23 cells enhanced levels of Smad2 phosphorylation and significantly suppressed cell proliferation in the presence of TGF-β, indicating that highly expressed TMEPAI suppresses levels of Smad phosphorylation in these cancer cells and reduces the growth inhibitory effects of TGF-β/Smad signaling.Together, these experiments indicate that TMEPAI promotes tumorigenic activities in lung cancer cells.
Affiliation: Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.Show MeSH
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Mentions: We further investigated the functional significance of TMEPAI in sphere-forming activities. The sphere-forming activities of TMEPAI knockdown cells were significantly reduced both in Calu3 (Fig.4a,b) and in NCI-H23 cells (Fig. S3d,e).
Affiliation: Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.