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Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.

Nishikawa R, Goto Y, Sakamoto S, Chiyomaru T, Enokida H, Kojima S, Kinoshita T, Yamamoto N, Nakagawa M, Naya Y, Ichikawa T, Seki N - Cancer Sci. (2014)

Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.LASP1 is a cytoskeletal scaffold protein that plays critical roles in cytoskeletal organization and cell migration.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.

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Effects of LASP1 downregulation by si-LASP1 on PCa cells (PC3 and DU145). (a) Cell proliferation determined with the XTT assay. (b) Cell migration activity determined with the wound healing assay. (c) Cell invasion activity determined with the Matrigel invasion assay. *P < 0.001.
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fig05: Effects of LASP1 downregulation by si-LASP1 on PCa cells (PC3 and DU145). (a) Cell proliferation determined with the XTT assay. (b) Cell migration activity determined with the wound healing assay. (c) Cell invasion activity determined with the Matrigel invasion assay. *P < 0.001.

Mentions: The XTT assay demonstrated that cell proliferation was not inhibited in si-LASP1 transfectants in comparison with the mock or miR-control transfectant cells (Fig. 5a). The Matrigel invasion assay demonstrated that cell invasion activity was significantly inhibited in si-LASP1 transfectants in comparison with the mock or negative control transfectant cells (Fig. 5b). The migration assay demonstrated that cell migration activity was significantly inhibited in si-LASP1 transfectants in comparison with the mock or negative control transfectant cells (Fig. 5c).


Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.

Nishikawa R, Goto Y, Sakamoto S, Chiyomaru T, Enokida H, Kojima S, Kinoshita T, Yamamoto N, Nakagawa M, Naya Y, Ichikawa T, Seki N - Cancer Sci. (2014)

Effects of LASP1 downregulation by si-LASP1 on PCa cells (PC3 and DU145). (a) Cell proliferation determined with the XTT assay. (b) Cell migration activity determined with the wound healing assay. (c) Cell invasion activity determined with the Matrigel invasion assay. *P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317931&req=5

fig05: Effects of LASP1 downregulation by si-LASP1 on PCa cells (PC3 and DU145). (a) Cell proliferation determined with the XTT assay. (b) Cell migration activity determined with the wound healing assay. (c) Cell invasion activity determined with the Matrigel invasion assay. *P < 0.001.
Mentions: The XTT assay demonstrated that cell proliferation was not inhibited in si-LASP1 transfectants in comparison with the mock or miR-control transfectant cells (Fig. 5a). The Matrigel invasion assay demonstrated that cell invasion activity was significantly inhibited in si-LASP1 transfectants in comparison with the mock or negative control transfectant cells (Fig. 5b). The migration assay demonstrated that cell migration activity was significantly inhibited in si-LASP1 transfectants in comparison with the mock or negative control transfectant cells (Fig. 5c).

Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.LASP1 is a cytoskeletal scaffold protein that plays critical roles in cytoskeletal organization and cell migration.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.

Show MeSH
Related in: MedlinePlus