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Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.

Nishikawa R, Goto Y, Sakamoto S, Chiyomaru T, Enokida H, Kojima S, Kinoshita T, Yamamoto N, Nakagawa M, Naya Y, Ichikawa T, Seki N - Cancer Sci. (2014)

Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.LASP1 is a cytoskeletal scaffold protein that plays critical roles in cytoskeletal organization and cell migration.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.

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LASP1 mRNA and LASP1 protein expression levels were suppressed by si-LASP1 transfection of DU145 and PC3 cells. (a) LASP1 mRNA expression 72 h after transfection with si-LASP1. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with si-LASP1. GAPDH was used as a loading control. The ratio of LASP1/GAPDH expression was evaluated using ImageJ software (ver. 1.43; http://rsbweb.nih.gov/ij/index.html).
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fig04: LASP1 mRNA and LASP1 protein expression levels were suppressed by si-LASP1 transfection of DU145 and PC3 cells. (a) LASP1 mRNA expression 72 h after transfection with si-LASP1. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with si-LASP1. GAPDH was used as a loading control. The ratio of LASP1/GAPDH expression was evaluated using ImageJ software (ver. 1.43; http://rsbweb.nih.gov/ij/index.html).

Mentions: To investigate the functional role of LASP1 in PCa cells, we performed loss-of-function studies using si-LASP1 transfectants. First, we evaluated the knockdown efficiency of si-LASP1 treatments in PC3 and DU145. Quantitative real-time RT-PCR and western blotting indicated that the siRNA effectively downregulated LASP1/LASP1 expression in both cell lines (Fig. 4).


Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.

Nishikawa R, Goto Y, Sakamoto S, Chiyomaru T, Enokida H, Kojima S, Kinoshita T, Yamamoto N, Nakagawa M, Naya Y, Ichikawa T, Seki N - Cancer Sci. (2014)

LASP1 mRNA and LASP1 protein expression levels were suppressed by si-LASP1 transfection of DU145 and PC3 cells. (a) LASP1 mRNA expression 72 h after transfection with si-LASP1. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with si-LASP1. GAPDH was used as a loading control. The ratio of LASP1/GAPDH expression was evaluated using ImageJ software (ver. 1.43; http://rsbweb.nih.gov/ij/index.html).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4317931&req=5

fig04: LASP1 mRNA and LASP1 protein expression levels were suppressed by si-LASP1 transfection of DU145 and PC3 cells. (a) LASP1 mRNA expression 72 h after transfection with si-LASP1. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with si-LASP1. GAPDH was used as a loading control. The ratio of LASP1/GAPDH expression was evaluated using ImageJ software (ver. 1.43; http://rsbweb.nih.gov/ij/index.html).
Mentions: To investigate the functional role of LASP1 in PCa cells, we performed loss-of-function studies using si-LASP1 transfectants. First, we evaluated the knockdown efficiency of si-LASP1 treatments in PC3 and DU145. Quantitative real-time RT-PCR and western blotting indicated that the siRNA effectively downregulated LASP1/LASP1 expression in both cell lines (Fig. 4).

Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.LASP1 is a cytoskeletal scaffold protein that plays critical roles in cytoskeletal organization and cell migration.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.

Show MeSH
Related in: MedlinePlus