Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.
Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.Luciferase reporter assays showed that miR-218 directly regulated expression of LASP1.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.
Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.Show MeSH
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Mentions: To investigate the functional role of LASP1 in PCa cells, we performed loss-of-function studies using si-LASP1 transfectants. First, we evaluated the knockdown efficiency of si-LASP1 treatments in PC3 and DU145. Quantitative real-time RT-PCR and western blotting indicated that the siRNA effectively downregulated LASP1/LASP1 expression in both cell lines (Fig. 4).
Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.