Limits...
Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.

Nishikawa R, Goto Y, Sakamoto S, Chiyomaru T, Enokida H, Kojima S, Kinoshita T, Yamamoto N, Nakagawa M, Naya Y, Ichikawa T, Seki N - Cancer Sci. (2014)

Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.Luciferase reporter assays showed that miR-218 directly regulated expression of LASP1.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.

Show MeSH

Related in: MedlinePlus

LASP1 expression was suppressed by miR-218 transfection of prostate cancer (PCa) cells. (a) LASP1 mRNA expression 72 h after transfection with miR-218. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with miR-218. GAPDH was used as a loading control. (c) miR-218 binding sites in the 3′-UTR of LASP1 mRNA. Luciferase reporter assays using three vectors encoding putative miR-218 target sites at positions 686–692, 1587–1593 and 2080–2087 for both wild-type and deletion (Del). Renilla luciferase values were normalized to firefly luciferase values, *P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317931&req=5

fig03: LASP1 expression was suppressed by miR-218 transfection of prostate cancer (PCa) cells. (a) LASP1 mRNA expression 72 h after transfection with miR-218. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with miR-218. GAPDH was used as a loading control. (c) miR-218 binding sites in the 3′-UTR of LASP1 mRNA. Luciferase reporter assays using three vectors encoding putative miR-218 target sites at positions 686–692, 1587–1593 and 2080–2087 for both wild-type and deletion (Del). Renilla luciferase values were normalized to firefly luciferase values, *P < 0.001.

Mentions: We performed quantitative real-time RT-PCR and western blotting in PC3 and DU145 cells to investigate whether LASP1 gene expression and LASP1 protein expression were reduced by restoration of miR-218. The mRNA and protein expression levels of LASP1/LASP1 were significantly repressed in miR-218 transfectants in comparison with mock or miR-control transfectants (Fig. 3a,b).


Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.

Nishikawa R, Goto Y, Sakamoto S, Chiyomaru T, Enokida H, Kojima S, Kinoshita T, Yamamoto N, Nakagawa M, Naya Y, Ichikawa T, Seki N - Cancer Sci. (2014)

LASP1 expression was suppressed by miR-218 transfection of prostate cancer (PCa) cells. (a) LASP1 mRNA expression 72 h after transfection with miR-218. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with miR-218. GAPDH was used as a loading control. (c) miR-218 binding sites in the 3′-UTR of LASP1 mRNA. Luciferase reporter assays using three vectors encoding putative miR-218 target sites at positions 686–692, 1587–1593 and 2080–2087 for both wild-type and deletion (Del). Renilla luciferase values were normalized to firefly luciferase values, *P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317931&req=5

fig03: LASP1 expression was suppressed by miR-218 transfection of prostate cancer (PCa) cells. (a) LASP1 mRNA expression 72 h after transfection with miR-218. GUSB expression was used for normalization. (b) LASP1 protein expression 72 h after transfection with miR-218. GAPDH was used as a loading control. (c) miR-218 binding sites in the 3′-UTR of LASP1 mRNA. Luciferase reporter assays using three vectors encoding putative miR-218 target sites at positions 686–692, 1587–1593 and 2080–2087 for both wild-type and deletion (Del). Renilla luciferase values were normalized to firefly luciferase values, *P < 0.001.
Mentions: We performed quantitative real-time RT-PCR and western blotting in PC3 and DU145 cells to investigate whether LASP1 gene expression and LASP1 protein expression were reduced by restoration of miR-218. The mRNA and protein expression levels of LASP1/LASP1 were significantly repressed in miR-218 transfectants in comparison with mock or miR-control transfectants (Fig. 3a,b).

Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.Luciferase reporter assays showed that miR-218 directly regulated expression of LASP1.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.

Show MeSH
Related in: MedlinePlus