Tumor-suppressive microRNA-218 inhibits cancer cell migration and invasion via targeting of LASP1 in prostate cancer.
Bottom Line: Our recent studies of the microRNA (miRNA) expression signature in prostate cancer (PCa) indicated that miRNA-218 (miR-218) was significantly downregulated in clinical specimens, suggesting that miR-218 might act as a tumor-suppressive miRNA in PCa.Luciferase reporter assays showed that miR-218 directly regulated expression of LASP1.Our data on pathways regulated by tumor-suppressive miR-218 provide new insight into the potential mechanisms of PCa oncogenesis and metastasis.
Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.Show MeSH
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Mentions: We performed quantitative real-time RT-PCR and western blotting in PC3 and DU145 cells to investigate whether LASP1 gene expression and LASP1 protein expression were reduced by restoration of miR-218. The mRNA and protein expression levels of LASP1/LASP1 were significantly repressed in miR-218 transfectants in comparison with mock or miR-control transfectants (Fig. 3a,b).
Affiliation: Department of Functional Genomics, Chiba, Japan; Department of Urology, Chiba University Graduate School of Medicine, Chiba, Japan.