Role of autophagy in high linear energy transfer radiation-induced cytotoxicity to tumor cells.
Bottom Line: Heavy-ion radiotherapy has a potential advantage over conventional radiotherapy due to improved dose distribution and a higher biological effectiveness in cancer therapy.The ability of carbon ions to inhibit the activation of the PI3K/Akt pathway rose with increasing their LET.Our data imply that targeting autophagy might enhance the effectiveness of heavy-ion radiotherapy.
Affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China.Show MeSH
Related in: MedlinePlus
Mentions: To quantify the possible induction of autophagy, we assayed the presence of acidic vesicular organelles, which are characteristic of this process and can be detected by flow cytometry in combination with AO staining. Shown in Figure 2 are the autophagy levels in HeLa, MCF-7, and MDA-MB-231 cells exposed to the carbon ions with LETs of 13 and 75 keV/μm at doses of 2 Gy or 5 Gy at 24, 48, and 72 h post-irradiation. Clearly, the autophagic rate of HeLa cells increased with LET and dose at the time points under investigation after the carbon ion irradiations. Similar results were also observed in MCF-7 and MDA-MB-231 cells.
Affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China.