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Role of autophagy in high linear energy transfer radiation-induced cytotoxicity to tumor cells.

Jin X, Liu Y, Ye F, Liu X, Furusawa Y, Wu Q, Li F, Zheng X, Dai Z, Li Q - Cancer Sci. (2014)

Bottom Line: Heavy-ion radiotherapy has a potential advantage over conventional radiotherapy due to improved dose distribution and a higher biological effectiveness in cancer therapy.Autophagy, as a novel important target to improve anticancer therapy, has recently attracted considerable attention.Our data imply that targeting autophagy might enhance the effectiveness of heavy-ion radiotherapy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China.

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Quantified assay of autophagy induced by high linear energy transfer carbon ions with flow cytometry in HeLa, MCF-7, and MDA-MB-231 cells. (a) Representative image of flow cytometry at 72 h after irradiation, where FL1-H (normal cells) and FL3-H (cells with autophagosomes) indicate green and red color intensities, respectively. Cells were irradiated with 75 keV/μm carbon ions at 5 Gy. (b) Statistical results of three independent experiments, where the white columns, gray columns, columns filled with sparse lines, columns filled with dense lines, and black columns represent the control groups, cells irradiated with 13 keV/μm carbons ions at 2 Gy, cells irradiated with 13 keV/μm carbon ions at 5 Gy, cells exposed to 75 keV/μm carbon ions at 2 Gy, and cells irradiated with 75 keV/μm carbon ions at 5 Gy, respectively. *P < 0.05, compared with control.
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fig02: Quantified assay of autophagy induced by high linear energy transfer carbon ions with flow cytometry in HeLa, MCF-7, and MDA-MB-231 cells. (a) Representative image of flow cytometry at 72 h after irradiation, where FL1-H (normal cells) and FL3-H (cells with autophagosomes) indicate green and red color intensities, respectively. Cells were irradiated with 75 keV/μm carbon ions at 5 Gy. (b) Statistical results of three independent experiments, where the white columns, gray columns, columns filled with sparse lines, columns filled with dense lines, and black columns represent the control groups, cells irradiated with 13 keV/μm carbons ions at 2 Gy, cells irradiated with 13 keV/μm carbon ions at 5 Gy, cells exposed to 75 keV/μm carbon ions at 2 Gy, and cells irradiated with 75 keV/μm carbon ions at 5 Gy, respectively. *P < 0.05, compared with control.

Mentions: To quantify the possible induction of autophagy, we assayed the presence of acidic vesicular organelles, which are characteristic of this process and can be detected by flow cytometry in combination with AO staining. Shown in Figure 2 are the autophagy levels in HeLa, MCF-7, and MDA-MB-231 cells exposed to the carbon ions with LETs of 13 and 75 keV/μm at doses of 2 Gy or 5 Gy at 24, 48, and 72 h post-irradiation. Clearly, the autophagic rate of HeLa cells increased with LET and dose at the time points under investigation after the carbon ion irradiations. Similar results were also observed in MCF-7 and MDA-MB-231 cells.


Role of autophagy in high linear energy transfer radiation-induced cytotoxicity to tumor cells.

Jin X, Liu Y, Ye F, Liu X, Furusawa Y, Wu Q, Li F, Zheng X, Dai Z, Li Q - Cancer Sci. (2014)

Quantified assay of autophagy induced by high linear energy transfer carbon ions with flow cytometry in HeLa, MCF-7, and MDA-MB-231 cells. (a) Representative image of flow cytometry at 72 h after irradiation, where FL1-H (normal cells) and FL3-H (cells with autophagosomes) indicate green and red color intensities, respectively. Cells were irradiated with 75 keV/μm carbon ions at 5 Gy. (b) Statistical results of three independent experiments, where the white columns, gray columns, columns filled with sparse lines, columns filled with dense lines, and black columns represent the control groups, cells irradiated with 13 keV/μm carbons ions at 2 Gy, cells irradiated with 13 keV/μm carbon ions at 5 Gy, cells exposed to 75 keV/μm carbon ions at 2 Gy, and cells irradiated with 75 keV/μm carbon ions at 5 Gy, respectively. *P < 0.05, compared with control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317928&req=5

fig02: Quantified assay of autophagy induced by high linear energy transfer carbon ions with flow cytometry in HeLa, MCF-7, and MDA-MB-231 cells. (a) Representative image of flow cytometry at 72 h after irradiation, where FL1-H (normal cells) and FL3-H (cells with autophagosomes) indicate green and red color intensities, respectively. Cells were irradiated with 75 keV/μm carbon ions at 5 Gy. (b) Statistical results of three independent experiments, where the white columns, gray columns, columns filled with sparse lines, columns filled with dense lines, and black columns represent the control groups, cells irradiated with 13 keV/μm carbons ions at 2 Gy, cells irradiated with 13 keV/μm carbon ions at 5 Gy, cells exposed to 75 keV/μm carbon ions at 2 Gy, and cells irradiated with 75 keV/μm carbon ions at 5 Gy, respectively. *P < 0.05, compared with control.
Mentions: To quantify the possible induction of autophagy, we assayed the presence of acidic vesicular organelles, which are characteristic of this process and can be detected by flow cytometry in combination with AO staining. Shown in Figure 2 are the autophagy levels in HeLa, MCF-7, and MDA-MB-231 cells exposed to the carbon ions with LETs of 13 and 75 keV/μm at doses of 2 Gy or 5 Gy at 24, 48, and 72 h post-irradiation. Clearly, the autophagic rate of HeLa cells increased with LET and dose at the time points under investigation after the carbon ion irradiations. Similar results were also observed in MCF-7 and MDA-MB-231 cells.

Bottom Line: Heavy-ion radiotherapy has a potential advantage over conventional radiotherapy due to improved dose distribution and a higher biological effectiveness in cancer therapy.Autophagy, as a novel important target to improve anticancer therapy, has recently attracted considerable attention.Our data imply that targeting autophagy might enhance the effectiveness of heavy-ion radiotherapy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China.

Show MeSH
Related in: MedlinePlus