Limits...
Role of autophagy in high linear energy transfer radiation-induced cytotoxicity to tumor cells.

Jin X, Liu Y, Ye F, Liu X, Furusawa Y, Wu Q, Li F, Zheng X, Dai Z, Li Q - Cancer Sci. (2014)

Bottom Line: Heavy-ion radiotherapy has a potential advantage over conventional radiotherapy due to improved dose distribution and a higher biological effectiveness in cancer therapy.Autophagy, as a novel important target to improve anticancer therapy, has recently attracted considerable attention.Our data imply that targeting autophagy might enhance the effectiveness of heavy-ion radiotherapy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China.

Show MeSH

Related in: MedlinePlus

Autophagy induced by carbon ions with linear energy transfer of 75 keV/μm and 2 Gy in HeLa, MCF-7, and MDA-MB-231 cells at morphological and molecular levels. (a) HeLa cells were stained with monodansylcadaverine at 24 h post-irradiation, then the cells were observed using fluorescence microscopy (arrowheads). (b) GFP-LC3 staining patterns were analyzed by fluorescence microscopy. (a,b) Scale bars = 10 μm. (c) LC3-II conversion and expression of sequestosome 1 (SQSTM1)/p62, Beclin 1, and Atg5 proteins in tumor cells following exposure to high-LET radiation; the relative level of LC3-II in comparison to β-actin is indicated below each immunoblot image.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317928&req=5

fig01: Autophagy induced by carbon ions with linear energy transfer of 75 keV/μm and 2 Gy in HeLa, MCF-7, and MDA-MB-231 cells at morphological and molecular levels. (a) HeLa cells were stained with monodansylcadaverine at 24 h post-irradiation, then the cells were observed using fluorescence microscopy (arrowheads). (b) GFP-LC3 staining patterns were analyzed by fluorescence microscopy. (a,b) Scale bars = 10 μm. (c) LC3-II conversion and expression of sequestosome 1 (SQSTM1)/p62, Beclin 1, and Atg5 proteins in tumor cells following exposure to high-LET radiation; the relative level of LC3-II in comparison to β-actin is indicated below each immunoblot image.

Mentions: First, the autophagic effect induced by the carbon ions with LET of 75 keV/μm in tumor cells was observed at the morphologic level. The MDC dye was used to assess levels of mature autophagic vesicle formation in HeLa cells following irradiation. Unirradiated cells (control) showed diffusive MDC staining instead of a punctate staining pattern. In contrast, the irradiated cells showed extensive punctate staining of bright blue, as shown in the Figure 1(a). Figure 1(b) shows that the redistribution of GFP-LC3 from a diffusive cytosolic to a punctate autophagosome associated pattern was observed in HeLa, MCF-7, and MDA-MB-231 cells at 4 and 24 h post-irradiation. Taken together, the morphological results support that the carbon ions induced autophagy in the tumor cells effectively.


Role of autophagy in high linear energy transfer radiation-induced cytotoxicity to tumor cells.

Jin X, Liu Y, Ye F, Liu X, Furusawa Y, Wu Q, Li F, Zheng X, Dai Z, Li Q - Cancer Sci. (2014)

Autophagy induced by carbon ions with linear energy transfer of 75 keV/μm and 2 Gy in HeLa, MCF-7, and MDA-MB-231 cells at morphological and molecular levels. (a) HeLa cells were stained with monodansylcadaverine at 24 h post-irradiation, then the cells were observed using fluorescence microscopy (arrowheads). (b) GFP-LC3 staining patterns were analyzed by fluorescence microscopy. (a,b) Scale bars = 10 μm. (c) LC3-II conversion and expression of sequestosome 1 (SQSTM1)/p62, Beclin 1, and Atg5 proteins in tumor cells following exposure to high-LET radiation; the relative level of LC3-II in comparison to β-actin is indicated below each immunoblot image.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317928&req=5

fig01: Autophagy induced by carbon ions with linear energy transfer of 75 keV/μm and 2 Gy in HeLa, MCF-7, and MDA-MB-231 cells at morphological and molecular levels. (a) HeLa cells were stained with monodansylcadaverine at 24 h post-irradiation, then the cells were observed using fluorescence microscopy (arrowheads). (b) GFP-LC3 staining patterns were analyzed by fluorescence microscopy. (a,b) Scale bars = 10 μm. (c) LC3-II conversion and expression of sequestosome 1 (SQSTM1)/p62, Beclin 1, and Atg5 proteins in tumor cells following exposure to high-LET radiation; the relative level of LC3-II in comparison to β-actin is indicated below each immunoblot image.
Mentions: First, the autophagic effect induced by the carbon ions with LET of 75 keV/μm in tumor cells was observed at the morphologic level. The MDC dye was used to assess levels of mature autophagic vesicle formation in HeLa cells following irradiation. Unirradiated cells (control) showed diffusive MDC staining instead of a punctate staining pattern. In contrast, the irradiated cells showed extensive punctate staining of bright blue, as shown in the Figure 1(a). Figure 1(b) shows that the redistribution of GFP-LC3 from a diffusive cytosolic to a punctate autophagosome associated pattern was observed in HeLa, MCF-7, and MDA-MB-231 cells at 4 and 24 h post-irradiation. Taken together, the morphological results support that the carbon ions induced autophagy in the tumor cells effectively.

Bottom Line: Heavy-ion radiotherapy has a potential advantage over conventional radiotherapy due to improved dose distribution and a higher biological effectiveness in cancer therapy.Autophagy, as a novel important target to improve anticancer therapy, has recently attracted considerable attention.Our data imply that targeting autophagy might enhance the effectiveness of heavy-ion radiotherapy.

View Article: PubMed Central - PubMed

Affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China; Key Laboratory of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou, China.

Show MeSH
Related in: MedlinePlus