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Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.

Ye X, Jiang F, Li Y, Mu J, Si L, Wang X, Ning S, Li Z - Cancer Sci. (2014)

Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

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Glabridin (GLA) attenuates the migratory and invasive capacities of MDA-MB-231 and BT-549 cells through miR-200c. After cells were transfected by anti-con or anti-miR-200c for 12 h, they were exposed to 0 or 10 μM GLA for 48 h. (a) Transwell analyses and numbers of migratory/invasive MDA-MB-231 (b) and BT-549 (c) cells (mean ± SD, n = 5). **P < 0.01 compared with medium control MDA-MB-231 or BT-549 cells, and ##P < 0.01 compared with anti-con-transfected MDA-MB-231 or BT-549 cells exposed to 10 μM GLA.
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fig07: Glabridin (GLA) attenuates the migratory and invasive capacities of MDA-MB-231 and BT-549 cells through miR-200c. After cells were transfected by anti-con or anti-miR-200c for 12 h, they were exposed to 0 or 10 μM GLA for 48 h. (a) Transwell analyses and numbers of migratory/invasive MDA-MB-231 (b) and BT-549 (c) cells (mean ± SD, n = 5). **P < 0.01 compared with medium control MDA-MB-231 or BT-549 cells, and ##P < 0.01 compared with anti-con-transfected MDA-MB-231 or BT-549 cells exposed to 10 μM GLA.

Mentions: Finally, the function of miR-200c in GLA-induced inhibition of migratory and invasive capacities in breast cancer cells was determined. GLA attenuated the migratory and invasive capacities of MDA-MB-231 (Fig. 7a,b) and BT-549 (Fig. 7a,c) cells; however, knockdown of miR-200c and miR-200a, specifically miR-200c, reversed such phenomena (Fig. S2).


Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.

Ye X, Jiang F, Li Y, Mu J, Si L, Wang X, Ning S, Li Z - Cancer Sci. (2014)

Glabridin (GLA) attenuates the migratory and invasive capacities of MDA-MB-231 and BT-549 cells through miR-200c. After cells were transfected by anti-con or anti-miR-200c for 12 h, they were exposed to 0 or 10 μM GLA for 48 h. (a) Transwell analyses and numbers of migratory/invasive MDA-MB-231 (b) and BT-549 (c) cells (mean ± SD, n = 5). **P < 0.01 compared with medium control MDA-MB-231 or BT-549 cells, and ##P < 0.01 compared with anti-con-transfected MDA-MB-231 or BT-549 cells exposed to 10 μM GLA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317926&req=5

fig07: Glabridin (GLA) attenuates the migratory and invasive capacities of MDA-MB-231 and BT-549 cells through miR-200c. After cells were transfected by anti-con or anti-miR-200c for 12 h, they were exposed to 0 or 10 μM GLA for 48 h. (a) Transwell analyses and numbers of migratory/invasive MDA-MB-231 (b) and BT-549 (c) cells (mean ± SD, n = 5). **P < 0.01 compared with medium control MDA-MB-231 or BT-549 cells, and ##P < 0.01 compared with anti-con-transfected MDA-MB-231 or BT-549 cells exposed to 10 μM GLA.
Mentions: Finally, the function of miR-200c in GLA-induced inhibition of migratory and invasive capacities in breast cancer cells was determined. GLA attenuated the migratory and invasive capacities of MDA-MB-231 (Fig. 7a,b) and BT-549 (Fig. 7a,c) cells; however, knockdown of miR-200c and miR-200a, specifically miR-200c, reversed such phenomena (Fig. S2).

Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

Show MeSH
Related in: MedlinePlus