Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.
Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.Show MeSH
Related in: MedlinePlus
Mentions: miR-200s are involved in regulating the EMT and cancer cell invasion through inhibition of the E-cadherin transcriptional repressors, ZEB1/2.(18) Here, in MDA-MB-231 cells exposed to GLA, there were increased expressions of miR-200a, miR-200c and miR-141 (Fig. 5a). For BT-549 cells, GLA elevated all miR-200s tested, except miR-429 (Fig. 5b). For both types of cells, GLA upregulated the expression of miR-200c more extensively. Moreover, GLA elevated the expressions of miR-200a, miR-200b, miR-200c, miR-141 and miR-429 in tumors formed by MDA-MB-231 cells (Fig. 4d).
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.