Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.
Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.Show MeSH
Related in: MedlinePlus
Mentions: MDA-MB-231 cells were injected s.c. into the right armpit of nude BALB/c mice. After 4 weeks, GLA (0 or 20 mg/kg·BW) in 100 μL corn oil was administered by gavage every day for another 5 weeks. Then, the tumor tissues were removed and used for RT-PCR, qRT-PCR and western blots. In tumors formed by these cells, GLA elevated expression of E-cadherin mRNA but decreased expression of vimentin mRNA (Fig. 4a,b). Western blots confirmed this observation; GLA enhanced E-cadherin protein expression but attenuated the expression of vimentin (Fig. 4c).
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.