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Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.

Ye X, Jiang F, Li Y, Mu J, Si L, Wang X, Ning S, Li Z - Cancer Sci. (2014)

Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

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Effects of glabridin (GLA) on epithelial–mesenchymal transition markers and miR-200s in MDA-MB-231 breast cancer xenografts. Nude mice bearing breast cancers were treated with the vehicle or with GLA (20 mg/kg/d). (a, top) RT-PCR analyses and (b) relative mRNA levels of E-cadherin and vimentin (mean ± SD, n = 3). (a, bottom) Western blot analyses and (c) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3). (d) Quantitative RT-PCR analysis of miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Con, control. **P < 0.01 compared with medium control MDA-MB-231 cells.
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fig04: Effects of glabridin (GLA) on epithelial–mesenchymal transition markers and miR-200s in MDA-MB-231 breast cancer xenografts. Nude mice bearing breast cancers were treated with the vehicle or with GLA (20 mg/kg/d). (a, top) RT-PCR analyses and (b) relative mRNA levels of E-cadherin and vimentin (mean ± SD, n = 3). (a, bottom) Western blot analyses and (c) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3). (d) Quantitative RT-PCR analysis of miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Con, control. **P < 0.01 compared with medium control MDA-MB-231 cells.

Mentions: MDA-MB-231 cells were injected s.c. into the right armpit of nude BALB/c mice. After 4 weeks, GLA (0 or 20 mg/kg·BW) in 100 μL corn oil was administered by gavage every day for another 5 weeks. Then, the tumor tissues were removed and used for RT-PCR, qRT-PCR and western blots. In tumors formed by these cells, GLA elevated expression of E-cadherin mRNA but decreased expression of vimentin mRNA (Fig. 4a,b). Western blots confirmed this observation; GLA enhanced E-cadherin protein expression but attenuated the expression of vimentin (Fig. 4c).


Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.

Ye X, Jiang F, Li Y, Mu J, Si L, Wang X, Ning S, Li Z - Cancer Sci. (2014)

Effects of glabridin (GLA) on epithelial–mesenchymal transition markers and miR-200s in MDA-MB-231 breast cancer xenografts. Nude mice bearing breast cancers were treated with the vehicle or with GLA (20 mg/kg/d). (a, top) RT-PCR analyses and (b) relative mRNA levels of E-cadherin and vimentin (mean ± SD, n = 3). (a, bottom) Western blot analyses and (c) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3). (d) Quantitative RT-PCR analysis of miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Con, control. **P < 0.01 compared with medium control MDA-MB-231 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317926&req=5

fig04: Effects of glabridin (GLA) on epithelial–mesenchymal transition markers and miR-200s in MDA-MB-231 breast cancer xenografts. Nude mice bearing breast cancers were treated with the vehicle or with GLA (20 mg/kg/d). (a, top) RT-PCR analyses and (b) relative mRNA levels of E-cadherin and vimentin (mean ± SD, n = 3). (a, bottom) Western blot analyses and (c) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3). (d) Quantitative RT-PCR analysis of miR-200a, miR-200b, miR-200c, miR-141 and miR-429. Con, control. **P < 0.01 compared with medium control MDA-MB-231 cells.
Mentions: MDA-MB-231 cells were injected s.c. into the right armpit of nude BALB/c mice. After 4 weeks, GLA (0 or 20 mg/kg·BW) in 100 μL corn oil was administered by gavage every day for another 5 weeks. Then, the tumor tissues were removed and used for RT-PCR, qRT-PCR and western blots. In tumors formed by these cells, GLA elevated expression of E-cadherin mRNA but decreased expression of vimentin mRNA (Fig. 4a,b). Western blots confirmed this observation; GLA enhanced E-cadherin protein expression but attenuated the expression of vimentin (Fig. 4c).

Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

Show MeSH
Related in: MedlinePlus