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Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.

Ye X, Jiang F, Li Y, Mu J, Si L, Wang X, Ning S, Li Z - Cancer Sci. (2014)

Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

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Effects of glabridin (GLA) on the epithelial–mesenchymal transition (EMT) markers in MDA-MB-231 and BT-549 cells. (a) Morphological images of MDA-MB-231 cells exposed to 0 or 10 μM GLA for 4 days. (b) MDA-MB-231 cells were exposed to 0, 10 or 20 μM GLA for 2 days. RT-PCR analyses and (c) relative mRNA levels of E-cadherin and vimentin were determined (mean ± SD, n = 3). MDA-MB-231 cells were exposed to 10 μM GLA for 0, 8, 16, 24 or 48 h. Western blot analyses (d) and relative protein levels (e) of E-cadherin and vimentin (mean ± SD, n = 3). BT-549 cells were exposed to 0, 1, 5, 10 or 20 μM GLA for 48 h. (f) Western blot analyses and (g) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3).
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fig03: Effects of glabridin (GLA) on the epithelial–mesenchymal transition (EMT) markers in MDA-MB-231 and BT-549 cells. (a) Morphological images of MDA-MB-231 cells exposed to 0 or 10 μM GLA for 4 days. (b) MDA-MB-231 cells were exposed to 0, 10 or 20 μM GLA for 2 days. RT-PCR analyses and (c) relative mRNA levels of E-cadherin and vimentin were determined (mean ± SD, n = 3). MDA-MB-231 cells were exposed to 10 μM GLA for 0, 8, 16, 24 or 48 h. Western blot analyses (d) and relative protein levels (e) of E-cadherin and vimentin (mean ± SD, n = 3). BT-549 cells were exposed to 0, 1, 5, 10 or 20 μM GLA for 48 h. (f) Western blot analyses and (g) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3).

Mentions: Because the EMT enables cells to move and invade,(17,18) the effects of GLA on this process were determined for breast cancer cells. MDA-MB-231 cells displayed a fibroblast-like mesenchymal appearance; however, after these cells were exposed to 10.0 μM GLA for 4 days, they showed an epithelial-like morphology (Fig. 3a). Moreover, in MDA-MB-231 cells, GLA enhanced the expression of E-cadherin mRNA and decreased the expression of vimentin mRNA, indicating transcriptional regulation (Fig. 3b,c). Furthermore, MDA-MB-231 cells were exposed to 10.0 μM GLA for different times, and the effects of GLA on the expressions of EMT/adhesive markers, E-cadherin and vimentin, were determined over 0–48 h. GLA elevated the E-cadherin levels but reduced the vimentin levels (Fig. 3d,e). For BT-549 cells, increasing concentrations of GLA elevated E-cadherin levels but decreased the expression of vimentin (Fig. 3f,g).


Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.

Ye X, Jiang F, Li Y, Mu J, Si L, Wang X, Ning S, Li Z - Cancer Sci. (2014)

Effects of glabridin (GLA) on the epithelial–mesenchymal transition (EMT) markers in MDA-MB-231 and BT-549 cells. (a) Morphological images of MDA-MB-231 cells exposed to 0 or 10 μM GLA for 4 days. (b) MDA-MB-231 cells were exposed to 0, 10 or 20 μM GLA for 2 days. RT-PCR analyses and (c) relative mRNA levels of E-cadherin and vimentin were determined (mean ± SD, n = 3). MDA-MB-231 cells were exposed to 10 μM GLA for 0, 8, 16, 24 or 48 h. Western blot analyses (d) and relative protein levels (e) of E-cadherin and vimentin (mean ± SD, n = 3). BT-549 cells were exposed to 0, 1, 5, 10 or 20 μM GLA for 48 h. (f) Western blot analyses and (g) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317926&req=5

fig03: Effects of glabridin (GLA) on the epithelial–mesenchymal transition (EMT) markers in MDA-MB-231 and BT-549 cells. (a) Morphological images of MDA-MB-231 cells exposed to 0 or 10 μM GLA for 4 days. (b) MDA-MB-231 cells were exposed to 0, 10 or 20 μM GLA for 2 days. RT-PCR analyses and (c) relative mRNA levels of E-cadherin and vimentin were determined (mean ± SD, n = 3). MDA-MB-231 cells were exposed to 10 μM GLA for 0, 8, 16, 24 or 48 h. Western blot analyses (d) and relative protein levels (e) of E-cadherin and vimentin (mean ± SD, n = 3). BT-549 cells were exposed to 0, 1, 5, 10 or 20 μM GLA for 48 h. (f) Western blot analyses and (g) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3).
Mentions: Because the EMT enables cells to move and invade,(17,18) the effects of GLA on this process were determined for breast cancer cells. MDA-MB-231 cells displayed a fibroblast-like mesenchymal appearance; however, after these cells were exposed to 10.0 μM GLA for 4 days, they showed an epithelial-like morphology (Fig. 3a). Moreover, in MDA-MB-231 cells, GLA enhanced the expression of E-cadherin mRNA and decreased the expression of vimentin mRNA, indicating transcriptional regulation (Fig. 3b,c). Furthermore, MDA-MB-231 cells were exposed to 10.0 μM GLA for different times, and the effects of GLA on the expressions of EMT/adhesive markers, E-cadherin and vimentin, were determined over 0–48 h. GLA elevated the E-cadherin levels but reduced the vimentin levels (Fig. 3d,e). For BT-549 cells, increasing concentrations of GLA elevated E-cadherin levels but decreased the expression of vimentin (Fig. 3f,g).

Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

Show MeSH
Related in: MedlinePlus