Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.
Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.Show MeSH
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Mentions: Because the EMT enables cells to move and invade,(17,18) the effects of GLA on this process were determined for breast cancer cells. MDA-MB-231 cells displayed a fibroblast-like mesenchymal appearance; however, after these cells were exposed to 10.0 μM GLA for 4 days, they showed an epithelial-like morphology (Fig. 3a). Moreover, in MDA-MB-231 cells, GLA enhanced the expression of E-cadherin mRNA and decreased the expression of vimentin mRNA, indicating transcriptional regulation (Fig. 3b,c). Furthermore, MDA-MB-231 cells were exposed to 10.0 μM GLA for different times, and the effects of GLA on the expressions of EMT/adhesive markers, E-cadherin and vimentin, were determined over 0–48 h. GLA elevated the E-cadherin levels but reduced the vimentin levels (Fig. 3d,e). For BT-549 cells, increasing concentrations of GLA elevated E-cadherin levels but decreased the expression of vimentin (Fig. 3f,g).
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.