Glabridin attenuates the migratory and invasive capacity of breast cancer cells by activating microRNA-200c.
Bottom Line: However, little is known regarding the effect of microRNA (miRNA) on GLA's anti-metastatic activity.GLA induced the mesenchymal-epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.Overexpression of miR-200c enhanced the expression of E-cadherin and decreased the expression of vimentin.
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.Show MeSH
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Mentions: Glabridin (GLA) did not appreciably affect the vitality of MDA-MB-231 or BT-549 breast cancer cells at concentrations of 0.0, 5.0, 10.0 or 20.0 μM (Fig. 1a). To determine the effects of GLA on the motility of breast cancer cells, wound healing assays were performed. MDA-MB-231 and BT-549 cells displayed high motility, but GLA reduced such capability in a dose-dependent manner (Fig. 1b). Because 10.0 μM GLA effectively decreased the motility of these cells, this concentration was selected for further investigations. As determined with transwell assays, GLA decreased both the migratory and invasive capacities of MDA-MB-231 and BT-549 cells (Fig. 2a–c).
Affiliation: Key Laboratory of Modern Toxicology, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.