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Size- and shape-dependent pleural translocation, deposition, fibrogenesis, and mesothelial proliferation by multiwalled carbon nanotubes.

Xu J, Alexander DB, Futakuchi M, Numano T, Fukamachi K, Suzui M, Omori T, Kanno J, Hirose A, Tsuda H - Cancer Sci. (2014)

Bottom Line: It was found that MWCNT-L, but not MWCNT-S, translocated into the pleural cavity, deposited in the parietal pleura, and induced fibrosis and patchy parietal mesothelial proliferation lesions.In contrast, MWCNT-S induced stronger inflammation and higher 8-hydroxydeoxyguanosine level in the lung tissue than MWCNT-L.These results suggest that MWCNT-L has higher risk of causing asbestos-like pleural lesions relevant to mesothelioma development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nanotoxicology Project, Nagoya City University, Nagoya, Japan; Department of Immunology, Anhui Medical University College of Basic Medical Sciences, Hefei, China.

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Demonstration of multiwalled carbon nanotube (MWCNT) fibers and analysis of inflammatory reactions in the pleural cavity. (a) H&E staining, polarized light microscopy (PLM), and SEM images of pleural cell pellets taken from rats treated with larger sized MWCNT (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). Arrows indicate MWCNT fibers. (b–d) Analysis of leukocyte number (b), proportion of CD 68-positive cells (c), and protein concentration (d) in the supernatants of pleural cavity lavages. ***P < 0.001 versus Pluronic F68 (PF68); #P < 0.05, ##P < 0.01, and ###P < 0.001 MWCNT-L versus MWCNT-S by two-tailed Student's t-test. NT, no treatment.
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fig04: Demonstration of multiwalled carbon nanotube (MWCNT) fibers and analysis of inflammatory reactions in the pleural cavity. (a) H&E staining, polarized light microscopy (PLM), and SEM images of pleural cell pellets taken from rats treated with larger sized MWCNT (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). Arrows indicate MWCNT fibers. (b–d) Analysis of leukocyte number (b), proportion of CD 68-positive cells (c), and protein concentration (d) in the supernatants of pleural cavity lavages. ***P < 0.001 versus Pluronic F68 (PF68); #P < 0.05, ##P < 0.01, and ###P < 0.001 MWCNT-L versus MWCNT-S by two-tailed Student's t-test. NT, no treatment.

Mentions: In the cell pellets of the PCL, MWCNT-L was found with both PLM and SEM observations. Larger sized MWCNT fibers were observed mainly within and/or attached to the cell surface of macrophages (Fig. 4a). The ratio of the MWCNT-L containing macrophages to the total cell count was approximately 1:1800 and the average length of MWCNT-L was 6.23 ± 4.11 μm (data not shown). Smaller sized MWCNT could not be detected (Fig. 4a).


Size- and shape-dependent pleural translocation, deposition, fibrogenesis, and mesothelial proliferation by multiwalled carbon nanotubes.

Xu J, Alexander DB, Futakuchi M, Numano T, Fukamachi K, Suzui M, Omori T, Kanno J, Hirose A, Tsuda H - Cancer Sci. (2014)

Demonstration of multiwalled carbon nanotube (MWCNT) fibers and analysis of inflammatory reactions in the pleural cavity. (a) H&E staining, polarized light microscopy (PLM), and SEM images of pleural cell pellets taken from rats treated with larger sized MWCNT (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). Arrows indicate MWCNT fibers. (b–d) Analysis of leukocyte number (b), proportion of CD 68-positive cells (c), and protein concentration (d) in the supernatants of pleural cavity lavages. ***P < 0.001 versus Pluronic F68 (PF68); #P < 0.05, ##P < 0.01, and ###P < 0.001 MWCNT-L versus MWCNT-S by two-tailed Student's t-test. NT, no treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317921&req=5

fig04: Demonstration of multiwalled carbon nanotube (MWCNT) fibers and analysis of inflammatory reactions in the pleural cavity. (a) H&E staining, polarized light microscopy (PLM), and SEM images of pleural cell pellets taken from rats treated with larger sized MWCNT (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). Arrows indicate MWCNT fibers. (b–d) Analysis of leukocyte number (b), proportion of CD 68-positive cells (c), and protein concentration (d) in the supernatants of pleural cavity lavages. ***P < 0.001 versus Pluronic F68 (PF68); #P < 0.05, ##P < 0.01, and ###P < 0.001 MWCNT-L versus MWCNT-S by two-tailed Student's t-test. NT, no treatment.
Mentions: In the cell pellets of the PCL, MWCNT-L was found with both PLM and SEM observations. Larger sized MWCNT fibers were observed mainly within and/or attached to the cell surface of macrophages (Fig. 4a). The ratio of the MWCNT-L containing macrophages to the total cell count was approximately 1:1800 and the average length of MWCNT-L was 6.23 ± 4.11 μm (data not shown). Smaller sized MWCNT could not be detected (Fig. 4a).

Bottom Line: It was found that MWCNT-L, but not MWCNT-S, translocated into the pleural cavity, deposited in the parietal pleura, and induced fibrosis and patchy parietal mesothelial proliferation lesions.In contrast, MWCNT-S induced stronger inflammation and higher 8-hydroxydeoxyguanosine level in the lung tissue than MWCNT-L.These results suggest that MWCNT-L has higher risk of causing asbestos-like pleural lesions relevant to mesothelioma development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nanotoxicology Project, Nagoya City University, Nagoya, Japan; Department of Immunology, Anhui Medical University College of Basic Medical Sciences, Hefei, China.

Show MeSH
Related in: MedlinePlus