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Size- and shape-dependent pleural translocation, deposition, fibrogenesis, and mesothelial proliferation by multiwalled carbon nanotubes.

Xu J, Alexander DB, Futakuchi M, Numano T, Fukamachi K, Suzui M, Omori T, Kanno J, Hirose A, Tsuda H - Cancer Sci. (2014)

Bottom Line: It was found that MWCNT-L, but not MWCNT-S, translocated into the pleural cavity, deposited in the parietal pleura, and induced fibrosis and patchy parietal mesothelial proliferation lesions.In contrast, MWCNT-S induced stronger inflammation and higher 8-hydroxydeoxyguanosine level in the lung tissue than MWCNT-L.These results suggest that MWCNT-L has higher risk of causing asbestos-like pleural lesions relevant to mesothelioma development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nanotoxicology Project, Nagoya City University, Nagoya, Japan; Department of Immunology, Anhui Medical University College of Basic Medical Sciences, Hefei, China.

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Related in: MedlinePlus

Cell proliferation of the parietal and visceral mesothelium. (a) Representative proliferating cell nuclear antigen (PCNA) immunostained images of the parietal and visceral pleural regions of rats treated with larger sized multiwalled carbon nanotubes (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). (b) PCNA indices (percentages of PCNA positive mesothelial cells in total mesothelial cells). Scale bar = 20 μm. **P < 0.01 versus Pluronic F68 (PF68); ††P < 0.01 MWCNT-L versus MWCNT-S.
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fig03: Cell proliferation of the parietal and visceral mesothelium. (a) Representative proliferating cell nuclear antigen (PCNA) immunostained images of the parietal and visceral pleural regions of rats treated with larger sized multiwalled carbon nanotubes (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). (b) PCNA indices (percentages of PCNA positive mesothelial cells in total mesothelial cells). Scale bar = 20 μm. **P < 0.01 versus Pluronic F68 (PF68); ††P < 0.01 MWCNT-L versus MWCNT-S.

Mentions: Neoplastic development was not found in the parietal or visceral pleura of either the MWCNT-L or MWCNT-S groups; however, in the MWCNT-L group, patchy foci of mesothelial cell proliferation were observed in the parietal pleura (Fig. 3a) and PCNA indices were significantly increased in both parietal and visceral mesothelium. The PCNA indices of the MWCNT-S group were comparable to those of the PF68 treated rats (Fig. 3b).


Size- and shape-dependent pleural translocation, deposition, fibrogenesis, and mesothelial proliferation by multiwalled carbon nanotubes.

Xu J, Alexander DB, Futakuchi M, Numano T, Fukamachi K, Suzui M, Omori T, Kanno J, Hirose A, Tsuda H - Cancer Sci. (2014)

Cell proliferation of the parietal and visceral mesothelium. (a) Representative proliferating cell nuclear antigen (PCNA) immunostained images of the parietal and visceral pleural regions of rats treated with larger sized multiwalled carbon nanotubes (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). (b) PCNA indices (percentages of PCNA positive mesothelial cells in total mesothelial cells). Scale bar = 20 μm. **P < 0.01 versus Pluronic F68 (PF68); ††P < 0.01 MWCNT-L versus MWCNT-S.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317921&req=5

fig03: Cell proliferation of the parietal and visceral mesothelium. (a) Representative proliferating cell nuclear antigen (PCNA) immunostained images of the parietal and visceral pleural regions of rats treated with larger sized multiwalled carbon nanotubes (MWCNT-L; l = 8 μm, d = 150 nm) or smaller sized MWCNT (MWCNT-S; l = 3 μm, d = 15 nm). (b) PCNA indices (percentages of PCNA positive mesothelial cells in total mesothelial cells). Scale bar = 20 μm. **P < 0.01 versus Pluronic F68 (PF68); ††P < 0.01 MWCNT-L versus MWCNT-S.
Mentions: Neoplastic development was not found in the parietal or visceral pleura of either the MWCNT-L or MWCNT-S groups; however, in the MWCNT-L group, patchy foci of mesothelial cell proliferation were observed in the parietal pleura (Fig. 3a) and PCNA indices were significantly increased in both parietal and visceral mesothelium. The PCNA indices of the MWCNT-S group were comparable to those of the PF68 treated rats (Fig. 3b).

Bottom Line: It was found that MWCNT-L, but not MWCNT-S, translocated into the pleural cavity, deposited in the parietal pleura, and induced fibrosis and patchy parietal mesothelial proliferation lesions.In contrast, MWCNT-S induced stronger inflammation and higher 8-hydroxydeoxyguanosine level in the lung tissue than MWCNT-L.These results suggest that MWCNT-L has higher risk of causing asbestos-like pleural lesions relevant to mesothelioma development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nanotoxicology Project, Nagoya City University, Nagoya, Japan; Department of Immunology, Anhui Medical University College of Basic Medical Sciences, Hefei, China.

Show MeSH
Related in: MedlinePlus