Limits...
Busulfan inhibits growth of human osteosarcoma through miR-200 family microRNAs in vitro and in vivo.

Mei Q, Li F, Quan H, Liu Y, Xu H - Cancer Sci. (2014)

Bottom Line: Busulfan has been widely used to treat CML.Moreover, a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 (miR-200) family which subsequently downregulated its target genes ZEB1 and ZEB2.Taken together, our data suggest that busulfan may have an anti-osteosarcoma effect through downregulating ZEB1 and ZEB2 through activating the miR-200 family, highlighting a possibility of using busulfan as a novel therapy for osteosarcoma.

View Article: PubMed Central - PubMed

Affiliation: 169th Hospital, School of Medicine, Hunan Normal University, Changsha, China.

Show MeSH

Related in: MedlinePlus

Busulfan upregulated the microRNA-200 (miR-200) family and modulated its target genes in osteosarcoma. (a,b) Messenger RNA from the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) was assayed by quantitative RT-PCR and normalized to control, showing that all were upregulated after busulfan treatment in U2OS (a) and MG-63 cells (b). (c) Dose-dependent and time-dependent effect of busulfan on the activation of miR-429. Busulfan (150 μM) seemed to be efficient for activation of miR-429. Moreover, efficient induction of miR-429 was detected 24 h after busulfan treatment. (d) Changes in mRNA of ZEB1 and ZEB2 in both lines were assayed by quantitative RT-PCR and normalized to control. *P < 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317920&req=5

fig02: Busulfan upregulated the microRNA-200 (miR-200) family and modulated its target genes in osteosarcoma. (a,b) Messenger RNA from the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) was assayed by quantitative RT-PCR and normalized to control, showing that all were upregulated after busulfan treatment in U2OS (a) and MG-63 cells (b). (c) Dose-dependent and time-dependent effect of busulfan on the activation of miR-429. Busulfan (150 μM) seemed to be efficient for activation of miR-429. Moreover, efficient induction of miR-429 was detected 24 h after busulfan treatment. (d) Changes in mRNA of ZEB1 and ZEB2 in both lines were assayed by quantitative RT-PCR and normalized to control. *P < 0.05

Mentions: All five members of the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) can inhibit epithelial–mesenchymal transition through activating E-cadherin transcription repressors ZEB1 and ZEB2. Thus, we measured the miRNA expression levels in U2OS and MG-63 cells. We found that the five members in the miRNA-200 family were all upregulated after busulfan treatment in both cell lines (Fig. 2a).


Busulfan inhibits growth of human osteosarcoma through miR-200 family microRNAs in vitro and in vivo.

Mei Q, Li F, Quan H, Liu Y, Xu H - Cancer Sci. (2014)

Busulfan upregulated the microRNA-200 (miR-200) family and modulated its target genes in osteosarcoma. (a,b) Messenger RNA from the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) was assayed by quantitative RT-PCR and normalized to control, showing that all were upregulated after busulfan treatment in U2OS (a) and MG-63 cells (b). (c) Dose-dependent and time-dependent effect of busulfan on the activation of miR-429. Busulfan (150 μM) seemed to be efficient for activation of miR-429. Moreover, efficient induction of miR-429 was detected 24 h after busulfan treatment. (d) Changes in mRNA of ZEB1 and ZEB2 in both lines were assayed by quantitative RT-PCR and normalized to control. *P < 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317920&req=5

fig02: Busulfan upregulated the microRNA-200 (miR-200) family and modulated its target genes in osteosarcoma. (a,b) Messenger RNA from the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) was assayed by quantitative RT-PCR and normalized to control, showing that all were upregulated after busulfan treatment in U2OS (a) and MG-63 cells (b). (c) Dose-dependent and time-dependent effect of busulfan on the activation of miR-429. Busulfan (150 μM) seemed to be efficient for activation of miR-429. Moreover, efficient induction of miR-429 was detected 24 h after busulfan treatment. (d) Changes in mRNA of ZEB1 and ZEB2 in both lines were assayed by quantitative RT-PCR and normalized to control. *P < 0.05
Mentions: All five members of the miR-200 family (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) can inhibit epithelial–mesenchymal transition through activating E-cadherin transcription repressors ZEB1 and ZEB2. Thus, we measured the miRNA expression levels in U2OS and MG-63 cells. We found that the five members in the miRNA-200 family were all upregulated after busulfan treatment in both cell lines (Fig. 2a).

Bottom Line: Busulfan has been widely used to treat CML.Moreover, a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 (miR-200) family which subsequently downregulated its target genes ZEB1 and ZEB2.Taken together, our data suggest that busulfan may have an anti-osteosarcoma effect through downregulating ZEB1 and ZEB2 through activating the miR-200 family, highlighting a possibility of using busulfan as a novel therapy for osteosarcoma.

View Article: PubMed Central - PubMed

Affiliation: 169th Hospital, School of Medicine, Hunan Normal University, Changsha, China.

Show MeSH
Related in: MedlinePlus