Novel regulatory program for norepinephrine-induced epithelial-mesenchymal transition in gastric adenocarcinoma cell lines.
Bottom Line: In this study, we show that NE does not only obviously induce EMT alterations in the morphological characteristics of gastric adenocarcinoma cells, but also increases the markers of EMT, including vimentin expression, and decreases E-cadherin expression, further resulting in cell motility and invasiveness.We also reveal that these actions are mainly mediated through the activation of β2 -AR-HIF-1α-Snail signaling pathways.In summary, this study implies that NE induces EMT in gastric adenocarcinoma through the regulation of β2 -AR-HIF-1α-Snail activity.
Affiliation: Department of General Surgery, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.Show MeSH
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Mentions: To further determine possible alterations in E-cadherin and vimentin, BGC-823 and SGC-7901 cells treated with NE underwent fluorescence immunostaining and were then analyzed by confocal microscopy. Results after 48 h indicated that the E-cadherin fluorescence signal in the NE group was lower than in the control group, whereas the vimentin fluorescence signal was substantially increased (Fig. 4). These results further suggest that NE has promotive effects on cellular EMT.
Affiliation: Department of General Surgery, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.