Novel regulatory program for norepinephrine-induced epithelial-mesenchymal transition in gastric adenocarcinoma cell lines.
Bottom Line: In this study, we show that NE does not only obviously induce EMT alterations in the morphological characteristics of gastric adenocarcinoma cells, but also increases the markers of EMT, including vimentin expression, and decreases E-cadherin expression, further resulting in cell motility and invasiveness.We also reveal that these actions are mainly mediated through the activation of β2 -AR-HIF-1α-Snail signaling pathways.In summary, this study implies that NE induces EMT in gastric adenocarcinoma through the regulation of β2 -AR-HIF-1α-Snail activity.
Affiliation: Department of General Surgery, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.Show MeSH
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Mentions: To determine whether stress hormones can induce EMT, we initially investigated the cell morphological changes of EMT by using optical and scanning electron microscopy in the gastric adenocarcinoma cells BGC-823 and SGC-7901 exposed to NE (10 μM; this concentration was chosen based on our previous study)(20) treatment. Both cell lines were treated with NE for 48 h. Figure 1 shows that the cancer cells underwent typical EMT morphological changes; specifically, the cells started to lose cell contacts, scattered from cell clusters, and acquired a spindle-shaped, fibroblast-like phenotype according to optical microscopy. Scanning electron microscopy results showed that the extracellular microvilli increased in some cells. These results suggest that NE can induce an occurrence of the EMT process.
Affiliation: Department of General Surgery, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, China.