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Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma.

Matsuwaki R, Ishii G, Zenke Y, Neri S, Aokage K, Hishida T, Yoshida J, Fujii S, Kondo H, Goya T, Nagai K, Ochiai A - Cancer Sci. (2014)

Bottom Line: Patients with mediastinal lymph node metastasis (N2) in squamous cell carcinoma (SqCC) of the lung have poor prognosis after surgical resection of the primary tumor.In the primary tumors, the expressions of all the examined molecules were not related to recurrence.Our study indicated that the immunophenotypes of both cancer cells and CAFs in metastatic lymph node tumors, but not primary tumors, provide useful information for predicting the recurrence of pathological N2 lung SqCC.

View Article: PubMed Central - PubMed

Affiliation: Division of Pathology, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa, Japan; Division of Thoracic Surgery, National Cancer Center Hospital East, Kashiwa, Japan; Division of Thoracic Surgery, Kyorin University, Tokyo, Japan.

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Staining with H&E and double immunofluorescence staining of tumor cells in a primary and a metastatic lymph node tumor in a patient with squamous cell carcinoma of the lung. (a,b) H&E staining in the primary tumor (a) and the metastatic lymph node tumor (b). Arrows indicate cancer-associated fibroblasts (CAFs). (c) Double immunofluorescence staining in the primary tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs). (d) Double immunofluorescence staining in the metastatic lymph node tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs).
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fig01: Staining with H&E and double immunofluorescence staining of tumor cells in a primary and a metastatic lymph node tumor in a patient with squamous cell carcinoma of the lung. (a,b) H&E staining in the primary tumor (a) and the metastatic lymph node tumor (b). Arrows indicate cancer-associated fibroblasts (CAFs). (c) Double immunofluorescence staining in the primary tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs). (d) Double immunofluorescence staining in the metastatic lymph node tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs).

Mentions: We confirmed that spindle cells had infiltrated the area around the cancer cells in the metastatic lymph node tumors, similar to the situation for the primary tumors (Fig. 1a,b). Double immunofluorescence staining revealed that the cancer cells were positive for AE1/3 (green) and that the spindle cells were positive for α-SMA (red), indicating that these cells were myofibroblasts (Fig. 1c,d). From these results, we confirmed that CAFs had also infiltrated the metastatic lymph node tumors, similar to the results of our previous study.(7)


Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma.

Matsuwaki R, Ishii G, Zenke Y, Neri S, Aokage K, Hishida T, Yoshida J, Fujii S, Kondo H, Goya T, Nagai K, Ochiai A - Cancer Sci. (2014)

Staining with H&E and double immunofluorescence staining of tumor cells in a primary and a metastatic lymph node tumor in a patient with squamous cell carcinoma of the lung. (a,b) H&E staining in the primary tumor (a) and the metastatic lymph node tumor (b). Arrows indicate cancer-associated fibroblasts (CAFs). (c) Double immunofluorescence staining in the primary tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs). (d) Double immunofluorescence staining in the metastatic lymph node tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317916&req=5

fig01: Staining with H&E and double immunofluorescence staining of tumor cells in a primary and a metastatic lymph node tumor in a patient with squamous cell carcinoma of the lung. (a,b) H&E staining in the primary tumor (a) and the metastatic lymph node tumor (b). Arrows indicate cancer-associated fibroblasts (CAFs). (c) Double immunofluorescence staining in the primary tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs). (d) Double immunofluorescence staining in the metastatic lymph node tumor. Blue, nucleus; green, AE1/3-positive cancer cells; red, α-smooth muscle actin-positive myofibroblasts (CAFs).
Mentions: We confirmed that spindle cells had infiltrated the area around the cancer cells in the metastatic lymph node tumors, similar to the situation for the primary tumors (Fig. 1a,b). Double immunofluorescence staining revealed that the cancer cells were positive for AE1/3 (green) and that the spindle cells were positive for α-SMA (red), indicating that these cells were myofibroblasts (Fig. 1c,d). From these results, we confirmed that CAFs had also infiltrated the metastatic lymph node tumors, similar to the results of our previous study.(7)

Bottom Line: Patients with mediastinal lymph node metastasis (N2) in squamous cell carcinoma (SqCC) of the lung have poor prognosis after surgical resection of the primary tumor.In the primary tumors, the expressions of all the examined molecules were not related to recurrence.Our study indicated that the immunophenotypes of both cancer cells and CAFs in metastatic lymph node tumors, but not primary tumors, provide useful information for predicting the recurrence of pathological N2 lung SqCC.

View Article: PubMed Central - PubMed

Affiliation: Division of Pathology, Research Center for Innovative Oncology, National Cancer Center Hospital East, Kashiwa, Japan; Division of Thoracic Surgery, National Cancer Center Hospital East, Kashiwa, Japan; Division of Thoracic Surgery, Kyorin University, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus