Expression of CD24 is associated with HER2 expression and supports HER2-Akt signaling in HER2-positive breast cancer cells.
Bottom Line: However, resistance to HER2-targeted therapy remains a major clinical problem.HER2-positive breast tumors are predominantly positive for CD24, suggesting that the expression of the two molecules is related.Flow cytometry thus revealed that the percentage of CD24-positive cells was markedly higher in the HER2-positive fraction than in the HER2-negative fraction.
Affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Tokyo, Japan; Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.Show MeSH
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Mentions: We injected 231-Luc, HER2-60 or HER2-90 cells (2 × 105) into a mammary fat pad of female nude mice in order to examine tumor growth in an orthotopic xenograft model. Tumors formed by HER2-60 or HER2-90 cells tended to be larger than those formed by 231-Luc cells, although the differences were not statistically significant (Fig. 3a,b). Immunohistochemical analysis of formalin-fixed tumor tissue revealed the proportionate overexpression of HER2 in tumors formed from HER2-60 or HER2-90 cells, whereas HER2 immunoreactivity was not detected in 231-Luc tumors (Fig. 3c).
Affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Tokyo, Japan; Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.