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Expression of CD24 is associated with HER2 expression and supports HER2-Akt signaling in HER2-positive breast cancer cells.

Hosonaga M, Arima Y, Sugihara E, Kohno N, Saya H - Cancer Sci. (2014)

Bottom Line: We found that expression of CD24 was increased by stable overexpression of HER2.Knockdown of CD24 also suppressed the phosphorylation of Akt, which functions downstream of HER2 and PI3K to promote cell survival.Our results thus indicate that CD24 supports both the expression of HER2 and the consequent activation of PI3K-Akt signaling.

View Article: PubMed Central - PubMed

Affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Tokyo, Japan; Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.

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Effect of overexpression of human epidermal growth factor receptor 2 (HER2) on tumor growth in an orthotopic xenograft model. (a) Bioluminescence imaging of nude mice injected with 231-Luc, HER2-60 or HER2-90 cells in a mammary fat pad at 4 weeks after cell injection. (b) Volume of orthotopic tumors determined at the indicated times after cell injection as in (a). Data are means ± SD for 8 to 10 mice per group. NS, not significant for comparison of 231-Luc cells with HER2-60 or HER2-90 cells (Student's t-test). (c) H&E staining and immunohistochemical analysis of HER2 expression in formalin-fixed, paraffin-embedded sections of orthotopic tumors formed at 3 to 4 weeks after cell injection. Scale bars, 200 μm.
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fig03: Effect of overexpression of human epidermal growth factor receptor 2 (HER2) on tumor growth in an orthotopic xenograft model. (a) Bioluminescence imaging of nude mice injected with 231-Luc, HER2-60 or HER2-90 cells in a mammary fat pad at 4 weeks after cell injection. (b) Volume of orthotopic tumors determined at the indicated times after cell injection as in (a). Data are means ± SD for 8 to 10 mice per group. NS, not significant for comparison of 231-Luc cells with HER2-60 or HER2-90 cells (Student's t-test). (c) H&E staining and immunohistochemical analysis of HER2 expression in formalin-fixed, paraffin-embedded sections of orthotopic tumors formed at 3 to 4 weeks after cell injection. Scale bars, 200 μm.

Mentions: We injected 231-Luc, HER2-60 or HER2-90 cells (2 × 105) into a mammary fat pad of female nude mice in order to examine tumor growth in an orthotopic xenograft model. Tumors formed by HER2-60 or HER2-90 cells tended to be larger than those formed by 231-Luc cells, although the differences were not statistically significant (Fig. 3a,b). Immunohistochemical analysis of formalin-fixed tumor tissue revealed the proportionate overexpression of HER2 in tumors formed from HER2-60 or HER2-90 cells, whereas HER2 immunoreactivity was not detected in 231-Luc tumors (Fig. 3c).


Expression of CD24 is associated with HER2 expression and supports HER2-Akt signaling in HER2-positive breast cancer cells.

Hosonaga M, Arima Y, Sugihara E, Kohno N, Saya H - Cancer Sci. (2014)

Effect of overexpression of human epidermal growth factor receptor 2 (HER2) on tumor growth in an orthotopic xenograft model. (a) Bioluminescence imaging of nude mice injected with 231-Luc, HER2-60 or HER2-90 cells in a mammary fat pad at 4 weeks after cell injection. (b) Volume of orthotopic tumors determined at the indicated times after cell injection as in (a). Data are means ± SD for 8 to 10 mice per group. NS, not significant for comparison of 231-Luc cells with HER2-60 or HER2-90 cells (Student's t-test). (c) H&E staining and immunohistochemical analysis of HER2 expression in formalin-fixed, paraffin-embedded sections of orthotopic tumors formed at 3 to 4 weeks after cell injection. Scale bars, 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317915&req=5

fig03: Effect of overexpression of human epidermal growth factor receptor 2 (HER2) on tumor growth in an orthotopic xenograft model. (a) Bioluminescence imaging of nude mice injected with 231-Luc, HER2-60 or HER2-90 cells in a mammary fat pad at 4 weeks after cell injection. (b) Volume of orthotopic tumors determined at the indicated times after cell injection as in (a). Data are means ± SD for 8 to 10 mice per group. NS, not significant for comparison of 231-Luc cells with HER2-60 or HER2-90 cells (Student's t-test). (c) H&E staining and immunohistochemical analysis of HER2 expression in formalin-fixed, paraffin-embedded sections of orthotopic tumors formed at 3 to 4 weeks after cell injection. Scale bars, 200 μm.
Mentions: We injected 231-Luc, HER2-60 or HER2-90 cells (2 × 105) into a mammary fat pad of female nude mice in order to examine tumor growth in an orthotopic xenograft model. Tumors formed by HER2-60 or HER2-90 cells tended to be larger than those formed by 231-Luc cells, although the differences were not statistically significant (Fig. 3a,b). Immunohistochemical analysis of formalin-fixed tumor tissue revealed the proportionate overexpression of HER2 in tumors formed from HER2-60 or HER2-90 cells, whereas HER2 immunoreactivity was not detected in 231-Luc tumors (Fig. 3c).

Bottom Line: We found that expression of CD24 was increased by stable overexpression of HER2.Knockdown of CD24 also suppressed the phosphorylation of Akt, which functions downstream of HER2 and PI3K to promote cell survival.Our results thus indicate that CD24 supports both the expression of HER2 and the consequent activation of PI3K-Akt signaling.

View Article: PubMed Central - PubMed

Affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Tokyo, Japan; Department of Breast Oncology, Tokyo Medical University, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus