Capn4 is a marker of poor clinical outcomes and promotes nasopharyngeal carcinoma metastasis via nuclear factor-κB-induced matrix metalloproteinase 2 expression.
Bottom Line: The patients with NPC displaying higher Capn4 had a significantly shorter overall survival (P = 0.002) and progression-free survival (P = 0.003).These events resulted from Capn4 downregulation were associated with reduced expression of matrix metalloproteinase 2 (MMP2), Snail, and Vimentin.Together, these findings argue a novel function of Capn4 in invasion and metastasis of NPC, and thereby suggest that Capn4 may represent an independent prognostic factor and a potential therapeutic target in NPC.
Affiliation: Department of Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.Show MeSH
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Mentions: To determine the functional role of Capn4 in metastasis of NPC, we first generated two clones of 5-8F cells stably transfected with Capn4 siRNA (Capn4/siRNA-1 and Capn4/siRNA-2). RT-PCR and Western blot analyses demonstrated that both protein and mRNA levels of Capn4 were substantially downregulated in Capn4/siRNA-1 and Capn4/siRNA-2 cells, compared to siRNA control cells (Fig.3a,b). Then, the transwell assays were performed to assess the effects of Capn4 knockdown on capabilities of invasion and migration in NPC cells. Notably, Capn4/siRNA cells displayed significantly lower rate of invasion and migration than the parental cells and siRNA control cells (Fig.3c). In addition, MTT assays revealed that knockdown of Capn4 also inhibited cell proliferation (Fig. S1), but failed to induce apoptosis (data not shown).
Affiliation: Department of Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.