Capn4 is a marker of poor clinical outcomes and promotes nasopharyngeal carcinoma metastasis via nuclear factor-κB-induced matrix metalloproteinase 2 expression.
Bottom Line: The patients with NPC displaying higher Capn4 had a significantly shorter overall survival (P = 0.002) and progression-free survival (P = 0.003).These events resulted from Capn4 downregulation were associated with reduced expression of matrix metalloproteinase 2 (MMP2), Snail, and Vimentin.Together, these findings argue a novel function of Capn4 in invasion and metastasis of NPC, and thereby suggest that Capn4 may represent an independent prognostic factor and a potential therapeutic target in NPC.
Affiliation: Department of Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.Show MeSH
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Mentions: To confirm the RT-PCR and Western blot results, we next dete-cted expression of Capn4 protein in archived paraffin-embedded NPC tumor and non-tumoral nasopharyngeal specimens by immunohistochemical staining. Whereas Capn4-specific staining was observed in both non-cancerous and malignant tissues, primarily localized in the cytoplasm of cells (Fig.2a), expression levels of Capn4 in NPC samples were significantly higher than that in non-tumoral tissues (P < 0.001; Table1).
Affiliation: Department of Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.