Capn4 is a marker of poor clinical outcomes and promotes nasopharyngeal carcinoma metastasis via nuclear factor-κB-induced matrix metalloproteinase 2 expression.
Bottom Line: The patients with NPC displaying higher Capn4 had a significantly shorter overall survival (P = 0.002) and progression-free survival (P = 0.003).These events resulted from Capn4 downregulation were associated with reduced expression of matrix metalloproteinase 2 (MMP2), Snail, and Vimentin.Together, these findings argue a novel function of Capn4 in invasion and metastasis of NPC, and thereby suggest that Capn4 may represent an independent prognostic factor and a potential therapeutic target in NPC.
Affiliation: Department of Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.Show MeSH
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Mentions: To examine expression of Capn4 in primary NPC tumor and normal nasopharyngeal tissues, RT-PCR and Western blot analysis were performed to detect mRNA and protein levels. The results revealed that the Capn4 mRNA and protein expression level was significantly upregulated in NPC tissues (n = 7) compared with normal nasopharyngeal tissues (n = 2; Fig.1a,b). In parallel, upregulation of Capn4 was also observed in three NPC cell lines, 5-8F, CNE2, and 6-10B, when compared to immortalized normal human nasopharyngeal epithelial cell lines NP69 (Fig.1c,d).
Affiliation: Department of Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.