LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.
Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.
Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.Show MeSH
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Mentions: To further confirm the role of JUB in promoting EMT in the opposite way, SW620, a derivative of the SW480 cell line and a highly metastatic CRC cell line, which showed a high expression of JUB (Fig.1b), was selected for depletion of the endogenous expression of JUB. Indeed, silencing of JUB repressed EMT in SW620 cells. Knockdown of JUB reduced expression of E-cadherin and enhanced expression of Vimentin, indicated by both western blotting and immunofluorescence staining assays (Fig.3a,b). The motility and invasive ability were also impaired by silencing of JUB as fewer cells were presented in the under-surface of the transwell chamber membrane (Fig.3c). Consistent with the data detailed above, 3-D culture showed that silencing JUB reduced the invasiveness of SW620 cells, exhibiting fewer or no outward projections (Fig.3d and Fig. S2b). Taken together, the abovementioned data strongly suggest that JUB promotes EMT in CRC.
Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.