Limits...
LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.

Liang XH, Zhang GX, Zeng YB, Yang HF, Li WH, Liu QL, Tang YL, He WG, Huang YN, Zhang L, Yu LN, Zeng XC - Cancer Sci. (2014)

Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.

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Ectopic expression of JUB induces epithelial–mesenchymal transition and enhances invasiveness in colorectal cancer cells. (a) Western blot analyses of E-cadherin and Vimnetin in the indicated cells. (b) Immunofluorescence staining of E-cadherin and Vimnetin in the indicated cells. DAPI was used to visualize nuclei. (c) Representative images (left panels) and quantification (right panel) of migrated and invaded SW480-JUB and vector cells. Data are the mean ± standard deviation (SD) from three independent experiments. (d) Representative images for 3-D culture of SW480-JUB and vector cells. All experiments were carried out at least three times. *P < 0.05 based on Student's t-test.
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fig02: Ectopic expression of JUB induces epithelial–mesenchymal transition and enhances invasiveness in colorectal cancer cells. (a) Western blot analyses of E-cadherin and Vimnetin in the indicated cells. (b) Immunofluorescence staining of E-cadherin and Vimnetin in the indicated cells. DAPI was used to visualize nuclei. (c) Representative images (left panels) and quantification (right panel) of migrated and invaded SW480-JUB and vector cells. Data are the mean ± standard deviation (SD) from three independent experiments. (d) Representative images for 3-D culture of SW480-JUB and vector cells. All experiments were carried out at least three times. *P < 0.05 based on Student's t-test.

Mentions: Snail was proved to repress E-cadherin expression to induce EMT.23,24 Previous reports have shown that JUB functions as a corepressor of Snail,21 but whether it could also induce EMT has not been considered. Thus, we investigated the role JUB in EMT. As shown in Figure 2(a), western blot analsis revealed that ectopic expression of JUB in SW480 cells significantly reduced the expression of the epithelial marker, indicated by E-cadherin, and enhanced the expression of the mesenchymal marker, indicated by Vimentin. This was further confirmed by immunofluorescence staining (Fig.2b).


LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.

Liang XH, Zhang GX, Zeng YB, Yang HF, Li WH, Liu QL, Tang YL, He WG, Huang YN, Zhang L, Yu LN, Zeng XC - Cancer Sci. (2014)

Ectopic expression of JUB induces epithelial–mesenchymal transition and enhances invasiveness in colorectal cancer cells. (a) Western blot analyses of E-cadherin and Vimnetin in the indicated cells. (b) Immunofluorescence staining of E-cadherin and Vimnetin in the indicated cells. DAPI was used to visualize nuclei. (c) Representative images (left panels) and quantification (right panel) of migrated and invaded SW480-JUB and vector cells. Data are the mean ± standard deviation (SD) from three independent experiments. (d) Representative images for 3-D culture of SW480-JUB and vector cells. All experiments were carried out at least three times. *P < 0.05 based on Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317901&req=5

fig02: Ectopic expression of JUB induces epithelial–mesenchymal transition and enhances invasiveness in colorectal cancer cells. (a) Western blot analyses of E-cadherin and Vimnetin in the indicated cells. (b) Immunofluorescence staining of E-cadherin and Vimnetin in the indicated cells. DAPI was used to visualize nuclei. (c) Representative images (left panels) and quantification (right panel) of migrated and invaded SW480-JUB and vector cells. Data are the mean ± standard deviation (SD) from three independent experiments. (d) Representative images for 3-D culture of SW480-JUB and vector cells. All experiments were carried out at least three times. *P < 0.05 based on Student's t-test.
Mentions: Snail was proved to repress E-cadherin expression to induce EMT.23,24 Previous reports have shown that JUB functions as a corepressor of Snail,21 but whether it could also induce EMT has not been considered. Thus, we investigated the role JUB in EMT. As shown in Figure 2(a), western blot analsis revealed that ectopic expression of JUB in SW480 cells significantly reduced the expression of the epithelial marker, indicated by E-cadherin, and enhanced the expression of the mesenchymal marker, indicated by Vimentin. This was further confirmed by immunofluorescence staining (Fig.2b).

Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.

Show MeSH
Related in: MedlinePlus