LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.
Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.
Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.Show MeSH
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Mentions: Snail was proved to repress E-cadherin expression to induce EMT.23,24 Previous reports have shown that JUB functions as a corepressor of Snail,21 but whether it could also induce EMT has not been considered. Thus, we investigated the role JUB in EMT. As shown in Figure 2(a), western blot analsis revealed that ectopic expression of JUB in SW480 cells significantly reduced the expression of the epithelial marker, indicated by E-cadherin, and enhanced the expression of the mesenchymal marker, indicated by Vimentin. This was further confirmed by immunofluorescence staining (Fig.2b).
Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.