LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.
Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.
Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.Show MeSH
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Mentions: Until now, the expression of Ajuba family proteins had not been explored. First, we analyzed their expression, including JUB, WTIP and LIMD1, in a publicly-available dataset (http://www.ncbi.nlm.nih.gov/gds/). As shown in Figure 1a, we were surprised to find that JUB, detected by different probes, but not the other two members of the Ajuba family proteins, was highly upregulated in tumor tissues compared to the matched adjacent normal tissues (GSE32323, n = 34). Furthermore, we detected the expression of Ajuba family proteins, E-cadherin and Vimentin (Fig. S1), in normal colorectal epithelial cells and CRC cell lines. Compared to the normal colorectal epithelial cells, JUB was significantly overexpressed in both protein and mRNA levels; however, the expression of WTIP and LIMD1 showed no obvious changes (Fig.1b,c).
Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.