Limits...
LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.

Liang XH, Zhang GX, Zeng YB, Yang HF, Li WH, Liu QL, Tang YL, He WG, Huang YN, Zhang L, Yu LN, Zeng XC - Cancer Sci. (2014)

Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.

Show MeSH

Related in: MedlinePlus

JUB is highly upregulated in colorectal cancer (CRC) specimens and cell lines. (a) Analysis of Ajuba family proteins transcriptional level in a published high-throughput microarray dataset (NCBI/GEO/GSE32323; n = 34). (b) Western blot analysis of the indicated proteins in CRC cell lines. β-actin was used as a loading control. (c) Real-time PCR analysis of Ajuba family proteins mRNA expression level. Expression data were normalized to β-actin and presented as mean ± standard deviation (SD) from three independent experiments. *P < 0.05 based on Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317901&req=5

fig01: JUB is highly upregulated in colorectal cancer (CRC) specimens and cell lines. (a) Analysis of Ajuba family proteins transcriptional level in a published high-throughput microarray dataset (NCBI/GEO/GSE32323; n = 34). (b) Western blot analysis of the indicated proteins in CRC cell lines. β-actin was used as a loading control. (c) Real-time PCR analysis of Ajuba family proteins mRNA expression level. Expression data were normalized to β-actin and presented as mean ± standard deviation (SD) from three independent experiments. *P < 0.05 based on Student's t-test.

Mentions: Until now, the expression of Ajuba family proteins had not been explored. First, we analyzed their expression, including JUB, WTIP and LIMD1, in a publicly-available dataset (http://www.ncbi.nlm.nih.gov/gds/). As shown in Figure 1a, we were surprised to find that JUB, detected by different probes, but not the other two members of the Ajuba family proteins, was highly upregulated in tumor tissues compared to the matched adjacent normal tissues (GSE32323, n = 34). Furthermore, we detected the expression of Ajuba family proteins, E-cadherin and Vimentin (Fig. S1), in normal colorectal epithelial cells and CRC cell lines. Compared to the normal colorectal epithelial cells, JUB was significantly overexpressed in both protein and mRNA levels; however, the expression of WTIP and LIMD1 showed no obvious changes (Fig.1b,c).


LIM protein JUB promotes epithelial-mesenchymal transition in colorectal cancer.

Liang XH, Zhang GX, Zeng YB, Yang HF, Li WH, Liu QL, Tang YL, He WG, Huang YN, Zhang L, Yu LN, Zeng XC - Cancer Sci. (2014)

JUB is highly upregulated in colorectal cancer (CRC) specimens and cell lines. (a) Analysis of Ajuba family proteins transcriptional level in a published high-throughput microarray dataset (NCBI/GEO/GSE32323; n = 34). (b) Western blot analysis of the indicated proteins in CRC cell lines. β-actin was used as a loading control. (c) Real-time PCR analysis of Ajuba family proteins mRNA expression level. Expression data were normalized to β-actin and presented as mean ± standard deviation (SD) from three independent experiments. *P < 0.05 based on Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317901&req=5

fig01: JUB is highly upregulated in colorectal cancer (CRC) specimens and cell lines. (a) Analysis of Ajuba family proteins transcriptional level in a published high-throughput microarray dataset (NCBI/GEO/GSE32323; n = 34). (b) Western blot analysis of the indicated proteins in CRC cell lines. β-actin was used as a loading control. (c) Real-time PCR analysis of Ajuba family proteins mRNA expression level. Expression data were normalized to β-actin and presented as mean ± standard deviation (SD) from three independent experiments. *P < 0.05 based on Student's t-test.
Mentions: Until now, the expression of Ajuba family proteins had not been explored. First, we analyzed their expression, including JUB, WTIP and LIMD1, in a publicly-available dataset (http://www.ncbi.nlm.nih.gov/gds/). As shown in Figure 1a, we were surprised to find that JUB, detected by different probes, but not the other two members of the Ajuba family proteins, was highly upregulated in tumor tissues compared to the matched adjacent normal tissues (GSE32323, n = 34). Furthermore, we detected the expression of Ajuba family proteins, E-cadherin and Vimentin (Fig. S1), in normal colorectal epithelial cells and CRC cell lines. Compared to the normal colorectal epithelial cells, JUB was significantly overexpressed in both protein and mRNA levels; however, the expression of WTIP and LIMD1 showed no obvious changes (Fig.1b,c).

Bottom Line: Here, we found that JUB, but not the other Ajuba family proteins, was highly upregulated in clinical specimens and CRC cell lines.The expression of JUB shows an inverse correlation with E-cadherin expression in clinical specimens.Thus, the LIM protein JUB may provide a novel target for therapy of metastatic CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Zengcheng People's Hospital (BoJi-Affiliated Hospital of Sun Yat-Sen University), Zengcheng, China.

Show MeSH
Related in: MedlinePlus