Anti-tumor effects of suberoylanilide hydroxamic acid on Epstein-Barr virus-associated T cell and natural killer cell lymphoma.
Bottom Line: SAHA suppressed the proliferation of T and NK cell lines, although no significant difference was observed between EBV-positive and EBV-negative cell lines.In addition, SAHA increased the expression of EBV-lytic genes and decreased the expression of EBV-latent genes.SAHA displayed a marked suppressive effect against EBV-associated T and NK cell lymphomas through either induction of apoptosis or cell cycle arrest, and may represent an alternative treatment option.
Affiliation: Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.Show MeSH
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Mentions: To determine whether apoptosis was induced by SAHA in the tested cell lines, early apoptotic cells were quantified by annexin V and 7-AAD staining. SAHA increased early apoptotic cells in the Jurkat, KAI3 and KHYG1 cell lines (Fig.3a). In other cell lines, the proportions of early apoptotic cells were not increased. Next, the cleavage of PARP was analyzed by immunoblotting. With the exception of the SNT16 cell line, SAHA induced the cleavage of PARP in the five cell lines (Fig.3b). Next, effects on the cell cycle were investigated. In the SNT16 and KAI3 cell lines, the population of cells in G1 phase was increased, whereas that in G2 phase was increased in the SNK6 cell line (Fig.4). In Jurkat and KHYG1 cells, the cell cycle assay was indeterminate because of the massive cell death caused by SAHA.
Affiliation: Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.