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Stathmin1 regulates p27 expression, proliferation and drug resistance, resulting in poor clinical prognosis in cholangiocarcinoma.

Watanabe A, Suzuki H, Yokobori T, Tsukagoshi M, Altan B, Kubo N, Suzuki S, Araki K, Wada S, Kashiwabara K, Hosouchi Y, Kuwano H - Cancer Sci. (2014)

Bottom Line: Patients in the high-STMN1-expression group were associated with shorter recurrence-free survival and overall survival than those in the low-expression group.STMN1 knockdown inhibited proliferation and increased the sensitivity of EHCC cells to paclitaxel.Understanding the regulation of p27 by STMN1 could provide new insights for overcoming therapeutic resistance in EHCC.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan.

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Immunohistochemical staining of stathmin1 (STMN1) and p27 in primary extrahepatic cholangiocarcinoma (EHCC) samples. (a) Low STMN1 expression in a primary EHCC specimen (original magnification, ×400). (b) High STMN1 expression in a primary EHCC specimen (original magnification, ×400). (c) High nuclear p27 expression and low cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). (d) Low nuclear p27 expression and high cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). Figures a, c and b, d show images from the same cases.
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fig01: Immunohistochemical staining of stathmin1 (STMN1) and p27 in primary extrahepatic cholangiocarcinoma (EHCC) samples. (a) Low STMN1 expression in a primary EHCC specimen (original magnification, ×400). (b) High STMN1 expression in a primary EHCC specimen (original magnification, ×400). (c) High nuclear p27 expression and low cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). (d) Low nuclear p27 expression and high cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). Figures a, c and b, d show images from the same cases.

Mentions: STMN1 expression was evaluated using immunohistochemistry in 80 EHCC samples. Thirty-three samples (41.2%) were negative for STMN1 expression (Fig.1a) and 47 samples (58.8%) were positive for cytoplasmic STMN1 expression (Fig.1b). Nuclear p27 expression was also evaluated in these samples. Twenty-five (31.2%) samples were positive for p27 expression (Fig.1c) and 55 samples (68.8%) were negative (Fig.1d). High STMN1 expression was associated with low p27 nuclear expression (P = 0.0011; Table1). Cytoplasmic p27 expression was evaluated in the same EHCC samples; 43 (54.4%) samples demonstrated high cytoplasmic staining for p27 expression (Fig.1d), whereas 36 (45.6%) samples exhibited low p27 expression (Fig.1c). High STMN1 expression was associated with high p27 cytoplasmic expression (P = 0.0063; Table1).


Stathmin1 regulates p27 expression, proliferation and drug resistance, resulting in poor clinical prognosis in cholangiocarcinoma.

Watanabe A, Suzuki H, Yokobori T, Tsukagoshi M, Altan B, Kubo N, Suzuki S, Araki K, Wada S, Kashiwabara K, Hosouchi Y, Kuwano H - Cancer Sci. (2014)

Immunohistochemical staining of stathmin1 (STMN1) and p27 in primary extrahepatic cholangiocarcinoma (EHCC) samples. (a) Low STMN1 expression in a primary EHCC specimen (original magnification, ×400). (b) High STMN1 expression in a primary EHCC specimen (original magnification, ×400). (c) High nuclear p27 expression and low cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). (d) Low nuclear p27 expression and high cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). Figures a, c and b, d show images from the same cases.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317896&req=5

fig01: Immunohistochemical staining of stathmin1 (STMN1) and p27 in primary extrahepatic cholangiocarcinoma (EHCC) samples. (a) Low STMN1 expression in a primary EHCC specimen (original magnification, ×400). (b) High STMN1 expression in a primary EHCC specimen (original magnification, ×400). (c) High nuclear p27 expression and low cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). (d) Low nuclear p27 expression and high cytoplasmic p27 expression in a primary EHCC specimen (original magnification, ×400). Figures a, c and b, d show images from the same cases.
Mentions: STMN1 expression was evaluated using immunohistochemistry in 80 EHCC samples. Thirty-three samples (41.2%) were negative for STMN1 expression (Fig.1a) and 47 samples (58.8%) were positive for cytoplasmic STMN1 expression (Fig.1b). Nuclear p27 expression was also evaluated in these samples. Twenty-five (31.2%) samples were positive for p27 expression (Fig.1c) and 55 samples (68.8%) were negative (Fig.1d). High STMN1 expression was associated with low p27 nuclear expression (P = 0.0011; Table1). Cytoplasmic p27 expression was evaluated in the same EHCC samples; 43 (54.4%) samples demonstrated high cytoplasmic staining for p27 expression (Fig.1d), whereas 36 (45.6%) samples exhibited low p27 expression (Fig.1c). High STMN1 expression was associated with high p27 cytoplasmic expression (P = 0.0063; Table1).

Bottom Line: Patients in the high-STMN1-expression group were associated with shorter recurrence-free survival and overall survival than those in the low-expression group.STMN1 knockdown inhibited proliferation and increased the sensitivity of EHCC cells to paclitaxel.Understanding the regulation of p27 by STMN1 could provide new insights for overcoming therapeutic resistance in EHCC.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, Gunma, Japan.

Show MeSH
Related in: MedlinePlus