Downregulation of DAB2IP results in cell proliferation and invasion and contributes to unfavorable outcomes in bladder cancer.
Bottom Line: Downregulation of DAB2IP could activate the ERK and Akt pathways and was correlated with the expression of epithelial-mesenchymal transition markers, such as E-cadherin and vimentin.In conclusion, downregulation of DAB2IP is associated with features of biologically aggressive UCB and results in cell proliferation, migration, and invasion of bladder cancer.DAB2IP may serve as a promising biomarker in patients with UCB treated by radical cystectomy and bilateral lymph node dissection.
Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.Show MeSH
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Mentions: To reveal the mechanism of DAB2IP function in these events, the activation status of Akt or ERK was determined based on the specific phosphorylation site of each protein. Compared with CON-siRNA cells, a dramatic activation of p-ERK1/2 and p-Akt was detected in DAB2IP-siRNA cells (Fig.5). These data showed that downregulation of DAB2IP could activate ERK or Akt in the presence of DAB2IP siRNA to knock down endogenous DAB2IP levels in bladder cancer cell lines.
Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.