Limits...
Downregulation of DAB2IP results in cell proliferation and invasion and contributes to unfavorable outcomes in bladder cancer.

Shen YJ, Kong ZL, Wan FN, Wang HK, Bian XJ, Gan HL, Wang CF, Ye DW - Cancer Sci. (2014)

Bottom Line: Downregulation of DAB2IP could activate the ERK and Akt pathways and was correlated with the expression of epithelial-mesenchymal transition markers, such as E-cadherin and vimentin.In conclusion, downregulation of DAB2IP is associated with features of biologically aggressive UCB and results in cell proliferation, migration, and invasion of bladder cancer.DAB2IP may serve as a promising biomarker in patients with UCB treated by radical cystectomy and bilateral lymph node dissection.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH

Related in: MedlinePlus

Increased Akt and ERK1/2 activation in T24 and 5637 bladder urothelial cancer cells transfected with specific siRNA (DAB2IP-siRNA) compared to negative control (CON-siRNA). Tissue lysates were analyzed by Western blot using DOC-2/DAB2 interactive protein (DAB2IP), phospho- (p-)Akt (S473), p-ERK1/2 (T202/Y204), total-Akt, and total-ERK1/2. Actin was used as a loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317890&req=5

fig05: Increased Akt and ERK1/2 activation in T24 and 5637 bladder urothelial cancer cells transfected with specific siRNA (DAB2IP-siRNA) compared to negative control (CON-siRNA). Tissue lysates were analyzed by Western blot using DOC-2/DAB2 interactive protein (DAB2IP), phospho- (p-)Akt (S473), p-ERK1/2 (T202/Y204), total-Akt, and total-ERK1/2. Actin was used as a loading control.

Mentions: To reveal the mechanism of DAB2IP function in these events, the activation status of Akt or ERK was determined based on the specific phosphorylation site of each protein. Compared with CON-siRNA cells, a dramatic activation of p-ERK1/2 and p-Akt was detected in DAB2IP-siRNA cells (Fig.5). These data showed that downregulation of DAB2IP could activate ERK or Akt in the presence of DAB2IP siRNA to knock down endogenous DAB2IP levels in bladder cancer cell lines.


Downregulation of DAB2IP results in cell proliferation and invasion and contributes to unfavorable outcomes in bladder cancer.

Shen YJ, Kong ZL, Wan FN, Wang HK, Bian XJ, Gan HL, Wang CF, Ye DW - Cancer Sci. (2014)

Increased Akt and ERK1/2 activation in T24 and 5637 bladder urothelial cancer cells transfected with specific siRNA (DAB2IP-siRNA) compared to negative control (CON-siRNA). Tissue lysates were analyzed by Western blot using DOC-2/DAB2 interactive protein (DAB2IP), phospho- (p-)Akt (S473), p-ERK1/2 (T202/Y204), total-Akt, and total-ERK1/2. Actin was used as a loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317890&req=5

fig05: Increased Akt and ERK1/2 activation in T24 and 5637 bladder urothelial cancer cells transfected with specific siRNA (DAB2IP-siRNA) compared to negative control (CON-siRNA). Tissue lysates were analyzed by Western blot using DOC-2/DAB2 interactive protein (DAB2IP), phospho- (p-)Akt (S473), p-ERK1/2 (T202/Y204), total-Akt, and total-ERK1/2. Actin was used as a loading control.
Mentions: To reveal the mechanism of DAB2IP function in these events, the activation status of Akt or ERK was determined based on the specific phosphorylation site of each protein. Compared with CON-siRNA cells, a dramatic activation of p-ERK1/2 and p-Akt was detected in DAB2IP-siRNA cells (Fig.5). These data showed that downregulation of DAB2IP could activate ERK or Akt in the presence of DAB2IP siRNA to knock down endogenous DAB2IP levels in bladder cancer cell lines.

Bottom Line: Downregulation of DAB2IP could activate the ERK and Akt pathways and was correlated with the expression of epithelial-mesenchymal transition markers, such as E-cadherin and vimentin.In conclusion, downregulation of DAB2IP is associated with features of biologically aggressive UCB and results in cell proliferation, migration, and invasion of bladder cancer.DAB2IP may serve as a promising biomarker in patients with UCB treated by radical cystectomy and bilateral lymph node dissection.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus