Downregulation of DAB2IP results in cell proliferation and invasion and contributes to unfavorable outcomes in bladder cancer.
Bottom Line: Downregulation of DAB2IP could activate the ERK and Akt pathways and was correlated with the expression of epithelial-mesenchymal transition markers, such as E-cadherin and vimentin.In conclusion, downregulation of DAB2IP is associated with features of biologically aggressive UCB and results in cell proliferation, migration, and invasion of bladder cancer.DAB2IP may serve as a promising biomarker in patients with UCB treated by radical cystectomy and bilateral lymph node dissection.
Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.Show MeSH
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Mentions: Endogenous levels of DAB2IP protein in T24 and 5637 were both knocked down using RNAi and confirmed by Western blot analysis (Fig.3a). Compared to cells transfected with CON-siRNA cells, no obvious change in cell morphology could be observed on DAB2IP-siRNA cells using RNAi (Fig.3b). However, DAB2IP-siRNA cells grew more quickly than CON-siRNA cells (P < 0.05; Fig.3c,d) accompanied with higher S-phase cell distribution. S-phase percentages for T24 cells: DAB2IP-siRNA versus CON-siRNA, 47.91 ± 1.03 versus 38.40 ± 0.83, P = 0.009; S-phase percentages for 5637 cells: DAB2IP-siRNA versus CON-siRNA, 53.81 ± 1.38 versus 38.77 ± 1.14, P = 0.007 (Fig.3e).
Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.