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Downregulation of DAB2IP results in cell proliferation and invasion and contributes to unfavorable outcomes in bladder cancer.

Shen YJ, Kong ZL, Wan FN, Wang HK, Bian XJ, Gan HL, Wang CF, Ye DW - Cancer Sci. (2014)

Bottom Line: Downregulation of DAB2IP could activate the ERK and Akt pathways and was correlated with the expression of epithelial-mesenchymal transition markers, such as E-cadherin and vimentin.In conclusion, downregulation of DAB2IP is associated with features of biologically aggressive UCB and results in cell proliferation, migration, and invasion of bladder cancer.DAB2IP may serve as a promising biomarker in patients with UCB treated by radical cystectomy and bilateral lymph node dissection.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

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Effects of DOC-2/DAB2 interactive protein (DAB2IP) silencing on cell proliferation and cell cycle in vitro of bladder urothelial cancer cells. (a) Knockdown of DAB2IP by specific siRNA was analyzed by Western blot. (b) Effect of DAB2IP depletion on cell morphology in T24 and 5637 cells transfected with specific siRNA (DAB2IP-siRNA) or negative control (CON-siRNA) under an inverted microscope (magnification, × 100). (c) Silencing endogenous DAB2IP promoted cell proliferation, as determined by MTT assay. Each bar represents the mean ± SD of three independent experiments. *P < 0.05. (d) Effect of DAB2IP depletion on the colony formation abilities of T24 and 5637 cells. Representative images are shown on the left, and quantification is shown on the right. The results are representative of three independent experiments, and the values shown are the mean ± SD. (e) Knockdown of DAB2IP resulted in more S-phase cell distribution. The population of cells in different phases was assessed by flow cytometric assay with propidium iodide staining and the percentages of cells in each phase are shown at the top right of each graph.
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fig03: Effects of DOC-2/DAB2 interactive protein (DAB2IP) silencing on cell proliferation and cell cycle in vitro of bladder urothelial cancer cells. (a) Knockdown of DAB2IP by specific siRNA was analyzed by Western blot. (b) Effect of DAB2IP depletion on cell morphology in T24 and 5637 cells transfected with specific siRNA (DAB2IP-siRNA) or negative control (CON-siRNA) under an inverted microscope (magnification, × 100). (c) Silencing endogenous DAB2IP promoted cell proliferation, as determined by MTT assay. Each bar represents the mean ± SD of three independent experiments. *P < 0.05. (d) Effect of DAB2IP depletion on the colony formation abilities of T24 and 5637 cells. Representative images are shown on the left, and quantification is shown on the right. The results are representative of three independent experiments, and the values shown are the mean ± SD. (e) Knockdown of DAB2IP resulted in more S-phase cell distribution. The population of cells in different phases was assessed by flow cytometric assay with propidium iodide staining and the percentages of cells in each phase are shown at the top right of each graph.

Mentions: Endogenous levels of DAB2IP protein in T24 and 5637 were both knocked down using RNAi and confirmed by Western blot analysis (Fig.3a). Compared to cells transfected with CON-siRNA cells, no obvious change in cell morphology could be observed on DAB2IP-siRNA cells using RNAi (Fig.3b). However, DAB2IP-siRNA cells grew more quickly than CON-siRNA cells (P < 0.05; Fig.3c,d) accompanied with higher S-phase cell distribution. S-phase percentages for T24 cells: DAB2IP-siRNA versus CON-siRNA, 47.91 ± 1.03 versus 38.40 ± 0.83, P = 0.009; S-phase percentages for 5637 cells: DAB2IP-siRNA versus CON-siRNA, 53.81 ± 1.38 versus 38.77 ± 1.14, P = 0.007 (Fig.3e).


Downregulation of DAB2IP results in cell proliferation and invasion and contributes to unfavorable outcomes in bladder cancer.

Shen YJ, Kong ZL, Wan FN, Wang HK, Bian XJ, Gan HL, Wang CF, Ye DW - Cancer Sci. (2014)

Effects of DOC-2/DAB2 interactive protein (DAB2IP) silencing on cell proliferation and cell cycle in vitro of bladder urothelial cancer cells. (a) Knockdown of DAB2IP by specific siRNA was analyzed by Western blot. (b) Effect of DAB2IP depletion on cell morphology in T24 and 5637 cells transfected with specific siRNA (DAB2IP-siRNA) or negative control (CON-siRNA) under an inverted microscope (magnification, × 100). (c) Silencing endogenous DAB2IP promoted cell proliferation, as determined by MTT assay. Each bar represents the mean ± SD of three independent experiments. *P < 0.05. (d) Effect of DAB2IP depletion on the colony formation abilities of T24 and 5637 cells. Representative images are shown on the left, and quantification is shown on the right. The results are representative of three independent experiments, and the values shown are the mean ± SD. (e) Knockdown of DAB2IP resulted in more S-phase cell distribution. The population of cells in different phases was assessed by flow cytometric assay with propidium iodide staining and the percentages of cells in each phase are shown at the top right of each graph.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317890&req=5

fig03: Effects of DOC-2/DAB2 interactive protein (DAB2IP) silencing on cell proliferation and cell cycle in vitro of bladder urothelial cancer cells. (a) Knockdown of DAB2IP by specific siRNA was analyzed by Western blot. (b) Effect of DAB2IP depletion on cell morphology in T24 and 5637 cells transfected with specific siRNA (DAB2IP-siRNA) or negative control (CON-siRNA) under an inverted microscope (magnification, × 100). (c) Silencing endogenous DAB2IP promoted cell proliferation, as determined by MTT assay. Each bar represents the mean ± SD of three independent experiments. *P < 0.05. (d) Effect of DAB2IP depletion on the colony formation abilities of T24 and 5637 cells. Representative images are shown on the left, and quantification is shown on the right. The results are representative of three independent experiments, and the values shown are the mean ± SD. (e) Knockdown of DAB2IP resulted in more S-phase cell distribution. The population of cells in different phases was assessed by flow cytometric assay with propidium iodide staining and the percentages of cells in each phase are shown at the top right of each graph.
Mentions: Endogenous levels of DAB2IP protein in T24 and 5637 were both knocked down using RNAi and confirmed by Western blot analysis (Fig.3a). Compared to cells transfected with CON-siRNA cells, no obvious change in cell morphology could be observed on DAB2IP-siRNA cells using RNAi (Fig.3b). However, DAB2IP-siRNA cells grew more quickly than CON-siRNA cells (P < 0.05; Fig.3c,d) accompanied with higher S-phase cell distribution. S-phase percentages for T24 cells: DAB2IP-siRNA versus CON-siRNA, 47.91 ± 1.03 versus 38.40 ± 0.83, P = 0.009; S-phase percentages for 5637 cells: DAB2IP-siRNA versus CON-siRNA, 53.81 ± 1.38 versus 38.77 ± 1.14, P = 0.007 (Fig.3e).

Bottom Line: Downregulation of DAB2IP could activate the ERK and Akt pathways and was correlated with the expression of epithelial-mesenchymal transition markers, such as E-cadherin and vimentin.In conclusion, downregulation of DAB2IP is associated with features of biologically aggressive UCB and results in cell proliferation, migration, and invasion of bladder cancer.DAB2IP may serve as a promising biomarker in patients with UCB treated by radical cystectomy and bilateral lymph node dissection.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus