Limits...
p53 accumulation is a strong predictor of recurrence in estrogen receptor-positive breast cancer patients treated with aromatase inhibitors.

Yamamoto M, Hosoda M, Nakano K, Jia S, Hatanaka KC, Takakuwa E, Hatanaka Y, Matsuno Y, Yamashita H - Cancer Sci. (2013)

Bottom Line: High p53 expression was positively correlated with tumor grade, HER2 score and Ki-67 expression.Significant association was observed between disease-free survival and high p53 expression in multivariate analysis (P < 0.0001).Compared with women without recurrence, women with early recurrence had significantly higher p53 expression (P < 0.0001), as did women with late recurrence (P = 0.037).

View Article: PubMed Central - PubMed

Affiliation: Breast and Endocrine Surgery, Hokkaido University Hospital, Sapporo, Japan.

Show MeSH

Related in: MedlinePlus

Immunohistochemical examination of p53 in various cancer cell lines using formalin-fixed, paraffin-embedded cell block materials. Representative results are shown. Negative or only weak staining in a few cells is observed in H1299 p53  cells and in H2228, Lovo, Hela and MCF-7 cells that do not have the TP53 gene mutation (upper panel), whereas nuclear staining can be observed in all cells in T47D, SK-BR-3, BT-474, MDA-MB-231 and MDA-MB-468 cells that have missense mutations of the TP53 gene (lower panel).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317887&req=5

fig02: Immunohistochemical examination of p53 in various cancer cell lines using formalin-fixed, paraffin-embedded cell block materials. Representative results are shown. Negative or only weak staining in a few cells is observed in H1299 p53 cells and in H2228, Lovo, Hela and MCF-7 cells that do not have the TP53 gene mutation (upper panel), whereas nuclear staining can be observed in all cells in T47D, SK-BR-3, BT-474, MDA-MB-231 and MDA-MB-468 cells that have missense mutations of the TP53 gene (lower panel).

Mentions: We first analyzed p53 expression by immunohistochemistry in a selection of cancer cell lines using formalin-fixed, paraffin-embedded cell block materials (Table2, Fig.2). Negative or week nuclear staining in only a few cells was observed in H1299 p53 cells and in H2228, Lovo, Hela and MCF-7 cells without the TP53 gene mutation (Fig.2, upper panel). In contrast, strong nuclear staining in all cells was seen in T47D, SK-BR-3, BT-474, MDA-MB-231 and MDA-MB-468 cells with missense mutations of the TP53 gene (Fig.2, lower panel).


p53 accumulation is a strong predictor of recurrence in estrogen receptor-positive breast cancer patients treated with aromatase inhibitors.

Yamamoto M, Hosoda M, Nakano K, Jia S, Hatanaka KC, Takakuwa E, Hatanaka Y, Matsuno Y, Yamashita H - Cancer Sci. (2013)

Immunohistochemical examination of p53 in various cancer cell lines using formalin-fixed, paraffin-embedded cell block materials. Representative results are shown. Negative or only weak staining in a few cells is observed in H1299 p53  cells and in H2228, Lovo, Hela and MCF-7 cells that do not have the TP53 gene mutation (upper panel), whereas nuclear staining can be observed in all cells in T47D, SK-BR-3, BT-474, MDA-MB-231 and MDA-MB-468 cells that have missense mutations of the TP53 gene (lower panel).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317887&req=5

fig02: Immunohistochemical examination of p53 in various cancer cell lines using formalin-fixed, paraffin-embedded cell block materials. Representative results are shown. Negative or only weak staining in a few cells is observed in H1299 p53 cells and in H2228, Lovo, Hela and MCF-7 cells that do not have the TP53 gene mutation (upper panel), whereas nuclear staining can be observed in all cells in T47D, SK-BR-3, BT-474, MDA-MB-231 and MDA-MB-468 cells that have missense mutations of the TP53 gene (lower panel).
Mentions: We first analyzed p53 expression by immunohistochemistry in a selection of cancer cell lines using formalin-fixed, paraffin-embedded cell block materials (Table2, Fig.2). Negative or week nuclear staining in only a few cells was observed in H1299 p53 cells and in H2228, Lovo, Hela and MCF-7 cells without the TP53 gene mutation (Fig.2, upper panel). In contrast, strong nuclear staining in all cells was seen in T47D, SK-BR-3, BT-474, MDA-MB-231 and MDA-MB-468 cells with missense mutations of the TP53 gene (Fig.2, lower panel).

Bottom Line: High p53 expression was positively correlated with tumor grade, HER2 score and Ki-67 expression.Significant association was observed between disease-free survival and high p53 expression in multivariate analysis (P < 0.0001).Compared with women without recurrence, women with early recurrence had significantly higher p53 expression (P < 0.0001), as did women with late recurrence (P = 0.037).

View Article: PubMed Central - PubMed

Affiliation: Breast and Endocrine Surgery, Hokkaido University Hospital, Sapporo, Japan.

Show MeSH
Related in: MedlinePlus