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Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells.

Uehara T, Kanazawa T, Mizukami H, Uchibori R, Tsukahara T, Urabe M, Kume A, Misawa K, Carey TE, Suzuki M, Ichimura K, Ozawa K - Cancer Sci. (2013)

Bottom Line: In this study, we first transduced HEp-2 and KB cell lines using a recombinant adeno-associated virus (rAAV)-green fluorescent protein (GFP) vector and confirmed a high GFP expression rate (>90%) in both cell lines at the standard vector dose.Additionally, the annexin V-positive rate and sub-G0/G1 phase population were significantly elevated in HEp-2 cells (mock vs GALR2: 12.3 vs 25.0% (P < 0.01) and 9.1 vs 32.0% (P < 0.05), respectively) after 48 h.Furthermore, in HEp-2 cells, GALR2-mediated apoptosis was caspase-independent, involving downregulation of ERK1/2, followed by induction of the pro-apoptotic Bcl-2 protein, Bim.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan; Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan; Department of Otolaryngology, Head and Neck Surgery, Jichi Medical University School of Medicine, Shimotsuke, Japan.

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Effects of mitochondrial apoptosis-associated proteins in HEp-2 cell co-administered with each of the recombinant adeno-associated virus (rAAV)-GALR vectors and galanin. Cox IV and β-actin were used as protein-loading controls for the mitochondrial and cytosolic fractions, respectively.
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fig08: Effects of mitochondrial apoptosis-associated proteins in HEp-2 cell co-administered with each of the recombinant adeno-associated virus (rAAV)-GALR vectors and galanin. Cox IV and β-actin were used as protein-loading controls for the mitochondrial and cytosolic fractions, respectively.

Mentions: We further examined whether activation of mitochondrial apoptosis-related factors were involved in initiating a caspase-independent pathway. Apoptosis inducers, such as apoptosis inducing factor (AIF), endonuclease G, HtrA2, and Bit1, are known to be released from the mitochondria to the cytosol and the nucleus by caspase-independent apoptotic signals. No translocation of these proteins in response to GALR2 activation (Fig.8) was seen in mitochondrial and cytosolic fractions from transduced HEp-2 cells cultured with/without galanin (1 μM) for 48 h.


Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells.

Uehara T, Kanazawa T, Mizukami H, Uchibori R, Tsukahara T, Urabe M, Kume A, Misawa K, Carey TE, Suzuki M, Ichimura K, Ozawa K - Cancer Sci. (2013)

Effects of mitochondrial apoptosis-associated proteins in HEp-2 cell co-administered with each of the recombinant adeno-associated virus (rAAV)-GALR vectors and galanin. Cox IV and β-actin were used as protein-loading controls for the mitochondrial and cytosolic fractions, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317884&req=5

fig08: Effects of mitochondrial apoptosis-associated proteins in HEp-2 cell co-administered with each of the recombinant adeno-associated virus (rAAV)-GALR vectors and galanin. Cox IV and β-actin were used as protein-loading controls for the mitochondrial and cytosolic fractions, respectively.
Mentions: We further examined whether activation of mitochondrial apoptosis-related factors were involved in initiating a caspase-independent pathway. Apoptosis inducers, such as apoptosis inducing factor (AIF), endonuclease G, HtrA2, and Bit1, are known to be released from the mitochondria to the cytosol and the nucleus by caspase-independent apoptotic signals. No translocation of these proteins in response to GALR2 activation (Fig.8) was seen in mitochondrial and cytosolic fractions from transduced HEp-2 cells cultured with/without galanin (1 μM) for 48 h.

Bottom Line: In this study, we first transduced HEp-2 and KB cell lines using a recombinant adeno-associated virus (rAAV)-green fluorescent protein (GFP) vector and confirmed a high GFP expression rate (>90%) in both cell lines at the standard vector dose.Additionally, the annexin V-positive rate and sub-G0/G1 phase population were significantly elevated in HEp-2 cells (mock vs GALR2: 12.3 vs 25.0% (P < 0.01) and 9.1 vs 32.0% (P < 0.05), respectively) after 48 h.Furthermore, in HEp-2 cells, GALR2-mediated apoptosis was caspase-independent, involving downregulation of ERK1/2, followed by induction of the pro-apoptotic Bcl-2 protein, Bim.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan; Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan; Department of Otolaryngology, Head and Neck Surgery, Jichi Medical University School of Medicine, Shimotsuke, Japan.

Show MeSH
Related in: MedlinePlus