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Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells.

Uehara T, Kanazawa T, Mizukami H, Uchibori R, Tsukahara T, Urabe M, Kume A, Misawa K, Carey TE, Suzuki M, Ichimura K, Ozawa K - Cancer Sci. (2013)

Bottom Line: In this study, we first transduced HEp-2 and KB cell lines using a recombinant adeno-associated virus (rAAV)-green fluorescent protein (GFP) vector and confirmed a high GFP expression rate (>90%) in both cell lines at the standard vector dose.Additionally, the annexin V-positive rate and sub-G0/G1 phase population were significantly elevated in HEp-2 cells (mock vs GALR2: 12.3 vs 25.0% (P < 0.01) and 9.1 vs 32.0% (P < 0.05), respectively) after 48 h.Furthermore, in HEp-2 cells, GALR2-mediated apoptosis was caspase-independent, involving downregulation of ERK1/2, followed by induction of the pro-apoptotic Bcl-2 protein, Bim.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan; Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan; Department of Otolaryngology, Head and Neck Surgery, Jichi Medical University School of Medicine, Shimotsuke, Japan.

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Co-administration of galanin and recombinant adeno-associated virus (rAAV)-GALR2 during cell culture inhibited cell proliferation and induced cell death in head and neck squamous cell carcinoma (HNSCC) cells. (a) Inhibitory effects of galanin on growth of HNSCC cells transduced with the respective rAAV vectors. (b) Flow cytometric analysis of cell apoptosis using the annexin V. (c) Cell cycle analysis by flow cytometry. *P < 0.05; **P < 0.01.
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fig04: Co-administration of galanin and recombinant adeno-associated virus (rAAV)-GALR2 during cell culture inhibited cell proliferation and induced cell death in head and neck squamous cell carcinoma (HNSCC) cells. (a) Inhibitory effects of galanin on growth of HNSCC cells transduced with the respective rAAV vectors. (b) Flow cytometric analysis of cell apoptosis using the annexin V. (c) Cell cycle analysis by flow cytometry. *P < 0.05; **P < 0.01.

Mentions: We examined the ability of each GALR signaling pathway to inhibit HNSCC growth. Culture of cells in the presence of both rAAV-GALR2 and varying doses of galanin in SFM for 24–72 h resulted in cell growth suppression in a time- and dose-dependent manner in both HNSCC lines, as assessed by WST-1 assay. After 72 h of stimulation, the cell growth rate was significantly decreased to 40% in HEp-2 and 60% in KB cells (Fig.4a). In contrast, co-administration of rAAV-GALR1 and galanin did not affect cell proliferation in either cell line (Fig.4a).


Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells.

Uehara T, Kanazawa T, Mizukami H, Uchibori R, Tsukahara T, Urabe M, Kume A, Misawa K, Carey TE, Suzuki M, Ichimura K, Ozawa K - Cancer Sci. (2013)

Co-administration of galanin and recombinant adeno-associated virus (rAAV)-GALR2 during cell culture inhibited cell proliferation and induced cell death in head and neck squamous cell carcinoma (HNSCC) cells. (a) Inhibitory effects of galanin on growth of HNSCC cells transduced with the respective rAAV vectors. (b) Flow cytometric analysis of cell apoptosis using the annexin V. (c) Cell cycle analysis by flow cytometry. *P < 0.05; **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317884&req=5

fig04: Co-administration of galanin and recombinant adeno-associated virus (rAAV)-GALR2 during cell culture inhibited cell proliferation and induced cell death in head and neck squamous cell carcinoma (HNSCC) cells. (a) Inhibitory effects of galanin on growth of HNSCC cells transduced with the respective rAAV vectors. (b) Flow cytometric analysis of cell apoptosis using the annexin V. (c) Cell cycle analysis by flow cytometry. *P < 0.05; **P < 0.01.
Mentions: We examined the ability of each GALR signaling pathway to inhibit HNSCC growth. Culture of cells in the presence of both rAAV-GALR2 and varying doses of galanin in SFM for 24–72 h resulted in cell growth suppression in a time- and dose-dependent manner in both HNSCC lines, as assessed by WST-1 assay. After 72 h of stimulation, the cell growth rate was significantly decreased to 40% in HEp-2 and 60% in KB cells (Fig.4a). In contrast, co-administration of rAAV-GALR1 and galanin did not affect cell proliferation in either cell line (Fig.4a).

Bottom Line: In this study, we first transduced HEp-2 and KB cell lines using a recombinant adeno-associated virus (rAAV)-green fluorescent protein (GFP) vector and confirmed a high GFP expression rate (>90%) in both cell lines at the standard vector dose.Additionally, the annexin V-positive rate and sub-G0/G1 phase population were significantly elevated in HEp-2 cells (mock vs GALR2: 12.3 vs 25.0% (P < 0.01) and 9.1 vs 32.0% (P < 0.05), respectively) after 48 h.Furthermore, in HEp-2 cells, GALR2-mediated apoptosis was caspase-independent, involving downregulation of ERK1/2, followed by induction of the pro-apoptotic Bcl-2 protein, Bim.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan; Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Japan; Department of Otolaryngology, Head and Neck Surgery, Jichi Medical University School of Medicine, Shimotsuke, Japan.

Show MeSH
Related in: MedlinePlus