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De novo diffuse large B-cell lymphoma with a CD20 immunohistochemistry-positive and flow cytometry-negative phenotype: molecular mechanisms and correlation with rituximab sensitivity.

Tokunaga T, Tomita A, Sugimoto K, Shimada K, Iriyama C, Hirose T, Shirahata-Adachi M, Suzuki Y, Mizuno H, Kiyoi H, Asano N, Nakamura S, Kinoshita T, Naoe T - Cancer Sci. (2013)

Bottom Line: Rituximab-mediated cytotoxicity in the CDC assay using IHC(+)/FCM(-) primary cells was significantly lower than in IHC(+)/FCM(+) cells (P < 0.05); however, partial effectiveness was confirmed.Lower CD20 expression with FCM does not rule out rituximab use in these patients if expression is confirmed with IHC.FCM using rituximab may be more informative than B1 for predicting rituximab effectiveness in IHC(+)/FCM(-) cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Department of Hematology/Oncology Research, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan.

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Prognosis of diffuse large B-cell lymphoma (DLBCL) patients with the CD20 IHC(+)/FCM(−) phenotype. (a) overall survival (OS) and (b) progression free survival (PFS) of DLBCL patients diagnosed in Nagoya University Hospital (n = 99). All patients were treated by combination chemotherapy with rituximab. These patients were classified by IPI, and the OS and PFS of each group are indicated in (c) and (d), respectively. (e) Comparison of OS of DLBCL patients who were diagnosed using both immunohistochemistry (IHC) and flow cytometry (FCM) (n = 36).
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fig05: Prognosis of diffuse large B-cell lymphoma (DLBCL) patients with the CD20 IHC(+)/FCM(−) phenotype. (a) overall survival (OS) and (b) progression free survival (PFS) of DLBCL patients diagnosed in Nagoya University Hospital (n = 99). All patients were treated by combination chemotherapy with rituximab. These patients were classified by IPI, and the OS and PFS of each group are indicated in (c) and (d), respectively. (e) Comparison of OS of DLBCL patients who were diagnosed using both immunohistochemistry (IHC) and flow cytometry (FCM) (n = 36).

Mentions: The OS rate was analyzed using Kaplan–Meier analysis (Fig.5). All DLBCL patients analyzed (n = 106) were treated with rituximab and CHOP-based combination chemotherapy at Nagoya University Hospital. The OS and PFS of all these patients at 3 years were 77% and 65.2%, respectively (Fig.5a,b). The OS and the PFS of each group classified by the IPI28 are indicated in Figure5(c,d). Patients with the IHC(+)/FCM(−) phenotype tended to show a lower survival rate than IHC(+)/FCM(+) patients, but no significant difference was found between these two groups (P = 0.664) (Fig.5e).


De novo diffuse large B-cell lymphoma with a CD20 immunohistochemistry-positive and flow cytometry-negative phenotype: molecular mechanisms and correlation with rituximab sensitivity.

Tokunaga T, Tomita A, Sugimoto K, Shimada K, Iriyama C, Hirose T, Shirahata-Adachi M, Suzuki Y, Mizuno H, Kiyoi H, Asano N, Nakamura S, Kinoshita T, Naoe T - Cancer Sci. (2013)

Prognosis of diffuse large B-cell lymphoma (DLBCL) patients with the CD20 IHC(+)/FCM(−) phenotype. (a) overall survival (OS) and (b) progression free survival (PFS) of DLBCL patients diagnosed in Nagoya University Hospital (n = 99). All patients were treated by combination chemotherapy with rituximab. These patients were classified by IPI, and the OS and PFS of each group are indicated in (c) and (d), respectively. (e) Comparison of OS of DLBCL patients who were diagnosed using both immunohistochemistry (IHC) and flow cytometry (FCM) (n = 36).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317883&req=5

fig05: Prognosis of diffuse large B-cell lymphoma (DLBCL) patients with the CD20 IHC(+)/FCM(−) phenotype. (a) overall survival (OS) and (b) progression free survival (PFS) of DLBCL patients diagnosed in Nagoya University Hospital (n = 99). All patients were treated by combination chemotherapy with rituximab. These patients were classified by IPI, and the OS and PFS of each group are indicated in (c) and (d), respectively. (e) Comparison of OS of DLBCL patients who were diagnosed using both immunohistochemistry (IHC) and flow cytometry (FCM) (n = 36).
Mentions: The OS rate was analyzed using Kaplan–Meier analysis (Fig.5). All DLBCL patients analyzed (n = 106) were treated with rituximab and CHOP-based combination chemotherapy at Nagoya University Hospital. The OS and PFS of all these patients at 3 years were 77% and 65.2%, respectively (Fig.5a,b). The OS and the PFS of each group classified by the IPI28 are indicated in Figure5(c,d). Patients with the IHC(+)/FCM(−) phenotype tended to show a lower survival rate than IHC(+)/FCM(+) patients, but no significant difference was found between these two groups (P = 0.664) (Fig.5e).

Bottom Line: Rituximab-mediated cytotoxicity in the CDC assay using IHC(+)/FCM(-) primary cells was significantly lower than in IHC(+)/FCM(+) cells (P < 0.05); however, partial effectiveness was confirmed.Lower CD20 expression with FCM does not rule out rituximab use in these patients if expression is confirmed with IHC.FCM using rituximab may be more informative than B1 for predicting rituximab effectiveness in IHC(+)/FCM(-) cases.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan; Department of Hematology/Oncology Research, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan.

Show MeSH
Related in: MedlinePlus