NRD1, which encodes nardilysin protein, promotes esophageal cancer cell invasion through induction of MMP2 and MMP3 expression.
Bottom Line: Furthermore, nardilysin expression was significantly associated with poorer prognosis (P = 0.0258).The invasiveness of NRD1-knockdown TE1 and TE5 esophageal cancer cell lines was less than that of the negative control siRNA-transfected cell lines.Expression of MMP2 and MMP3 mRNA was significantly lower in NRD1-knockdown TE5 cells than in negative control siRNA-transfected cells.
Affiliation: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.Show MeSH
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Mentions: To identify novel biomarkers or therapeutic targets for ESCC, we reviewed a list of genes with upregulated expression in ESCC compared with normal esophagus, as identified by our SAGE analysis.9 Among the 30 most upregulated genes in ESCC, we focused on the NRD1 gene, which encodes the nardilysin protein, because expression of NRD1 has not been investigated in ESCC, and an antibody against nardilysin is commercially available. We first performed qRT-PCR analysis of NRD1 in 15 types of normal tissue samples, six esophageal cancer cell lines, and two ESCC tissue samples (Fig.1a). Among the various normal tissue samples, abundant NRD1 expression was found in normal pancreas and skeletal muscle, as reported previously.11 Expression of NRD1 in these normal tissue samples was greatest in skeletal muscle; however, expression of NRD1 in ESCC tissue samples was even greater than in skeletal muscle.
Affiliation: Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.