Ganglioside GD3 induces convergence and synergism of adhesion and hepatocyte growth factor/Met signals in melanomas.
Bottom Line: In this study, we analyzed the effects of GD3 expression on cell signals triggered by hepatocyte growth factor (HGF)/Met interaction and by adhesion to collagen type I (CL-I).When resistance to induced apoptosis by H2O2 was examined, only GD3+ cells treated with both HGF and adhesion to CL-I showed clearly low percentages of dead cells compared with GD3- cells or GD3+ cells treated with either one of the stimulants.Cell growth measured by 5-ethynyl-2' deoxyuridine uptake also showed synergistic effects in GD3+ cells.
Affiliation: Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai, Japan; Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.Show MeSH
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Mentions: To examine the implications of enhanced Erk activation in cell proliferation, we carried out the EdU uptake assay. The percentage of EdU positive cells was higher in GD3+ cells than in GD3− cells after 21 h of costimulation with HGF and adhesion to CL-I (Fig.8A). The percentage of EdU positive cells was also higher in GD3+ cells than in GD3− cells after 21 h of stimulation with adhesion to CL-I alone, whereas HGF treatment showed no significant differences in the ratios of EdU positive cells between GD3+ and GD3− cells (Fig.8B). When cells were costimulated by HGF and adhesion to CL-I, the percentage of positive cells in GD3+ cells was clearly higher than that in GD3− cells, and also than that of GD3+ cells stimulated by CL-I alone; no clear difference was found in GD3− cells between the CL-I-treated and CL-I/HGF-treated groups (Fig.8B). These results suggested that GD3 expression promotes cell proliferation with costimulation using HGF and adhesion to CL-I.
Affiliation: Department of Biomedical Sciences, Chubu University College of Life and Health Sciences, Kasugai, Japan; Department of Biochemistry II, Nagoya University Graduate School of Medicine, Nagoya, Japan.