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Downregulation of miR-106b induced breast cancer cell invasion and motility in association with overexpression of matrix metalloproteinase 2.

Ni X, Xia T, Zhao Y, Zhou W, Wu N, Liu X, Ding Q, Zha X, Sha J, Wang S - Cancer Sci. (2013)

Bottom Line: MMP2 was shown to be a direct target of miR-106b.Both gain- and loss-of-function studies showed that MMP2 could promote the migration and invasion of BC cells and that miR-106b could suppress both.The blockage of MMP2 by RNA interference mimicked the anti-migration and anti-invasion effects of miR-106b, and introduction of MMP2 antagonized the function of miR-106b.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.

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Effects of miR-106b on aggressive behavior of SUM1315-bo and MCF-7 cells in vitro. (a) miR-106b mimic increased miR-106b level in SUM1315-bo cells. (b) Transwell invasion (n = 3) and migration (n = 3) assays showed that SUM1315-bo cells transfected with miR-106b mimic (200 nM) decreased the invasive and migratory ability of the cells. Cells were counted after staining with crystal violet. Representative images are shown at left. Graphs indicate the average number of cells per field 24 h after transfection. Data represent the mean ± standard deviation (SD) of at least three independent experiments. *P < 0.05, **P < 0.01. Magnification in b–c, ×100. (c) Suppression of miR-106b by specific inhibitor in MCF-7 cells. Mature miR-106b was quantified using miRNA-specific real-time polymerase chain reaction (PCR) using U6 RNA for normalization. (d) Transwell invasion and migration assays on MCF-7 cells indicated that the downregulation of miR-106b increased the invasive and migratory ability of the cells. Data represent the mean ± SD of at least three independent experiments *P < 0.05, **P < 0.01.
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fig03: Effects of miR-106b on aggressive behavior of SUM1315-bo and MCF-7 cells in vitro. (a) miR-106b mimic increased miR-106b level in SUM1315-bo cells. (b) Transwell invasion (n = 3) and migration (n = 3) assays showed that SUM1315-bo cells transfected with miR-106b mimic (200 nM) decreased the invasive and migratory ability of the cells. Cells were counted after staining with crystal violet. Representative images are shown at left. Graphs indicate the average number of cells per field 24 h after transfection. Data represent the mean ± standard deviation (SD) of at least three independent experiments. *P < 0.05, **P < 0.01. Magnification in b–c, ×100. (c) Suppression of miR-106b by specific inhibitor in MCF-7 cells. Mature miR-106b was quantified using miRNA-specific real-time polymerase chain reaction (PCR) using U6 RNA for normalization. (d) Transwell invasion and migration assays on MCF-7 cells indicated that the downregulation of miR-106b increased the invasive and migratory ability of the cells. Data represent the mean ± SD of at least three independent experiments *P < 0.05, **P < 0.01.

Mentions: Because SUM1315-bo cells have higher migratory potential than MCF-7 cells, we speculated that miR-106b might be involved in BC migration. To confirm this hypothesis, we transfected SUM1315-bo cells with 200 nM miR-106b mimic to determine whether overexpression of miR-106b would decrease migration and invasion. Using miRNA-specific quantitative real-time PCR, we found that the level of mature miR-106b had increased by 10.55-fold after transfection (Fig.3a). We further analyzed the effects of miR-106b on the migratory and invasive behavior of SUM1315-bo cells. Results showed a 1.83-fold decrease in cell motility and a 5.01-fold decrease in cell invasiveness, respectively, after transfection of the miR-106b mimic (Fig.3b). These results indicate that overexpression of miR-106b can inhibit migration and invasion of SUM1315-bo cells in vitro to a considerable extent.


Downregulation of miR-106b induced breast cancer cell invasion and motility in association with overexpression of matrix metalloproteinase 2.

Ni X, Xia T, Zhao Y, Zhou W, Wu N, Liu X, Ding Q, Zha X, Sha J, Wang S - Cancer Sci. (2013)

Effects of miR-106b on aggressive behavior of SUM1315-bo and MCF-7 cells in vitro. (a) miR-106b mimic increased miR-106b level in SUM1315-bo cells. (b) Transwell invasion (n = 3) and migration (n = 3) assays showed that SUM1315-bo cells transfected with miR-106b mimic (200 nM) decreased the invasive and migratory ability of the cells. Cells were counted after staining with crystal violet. Representative images are shown at left. Graphs indicate the average number of cells per field 24 h after transfection. Data represent the mean ± standard deviation (SD) of at least three independent experiments. *P < 0.05, **P < 0.01. Magnification in b–c, ×100. (c) Suppression of miR-106b by specific inhibitor in MCF-7 cells. Mature miR-106b was quantified using miRNA-specific real-time polymerase chain reaction (PCR) using U6 RNA for normalization. (d) Transwell invasion and migration assays on MCF-7 cells indicated that the downregulation of miR-106b increased the invasive and migratory ability of the cells. Data represent the mean ± SD of at least three independent experiments *P < 0.05, **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4317878&req=5

fig03: Effects of miR-106b on aggressive behavior of SUM1315-bo and MCF-7 cells in vitro. (a) miR-106b mimic increased miR-106b level in SUM1315-bo cells. (b) Transwell invasion (n = 3) and migration (n = 3) assays showed that SUM1315-bo cells transfected with miR-106b mimic (200 nM) decreased the invasive and migratory ability of the cells. Cells were counted after staining with crystal violet. Representative images are shown at left. Graphs indicate the average number of cells per field 24 h after transfection. Data represent the mean ± standard deviation (SD) of at least three independent experiments. *P < 0.05, **P < 0.01. Magnification in b–c, ×100. (c) Suppression of miR-106b by specific inhibitor in MCF-7 cells. Mature miR-106b was quantified using miRNA-specific real-time polymerase chain reaction (PCR) using U6 RNA for normalization. (d) Transwell invasion and migration assays on MCF-7 cells indicated that the downregulation of miR-106b increased the invasive and migratory ability of the cells. Data represent the mean ± SD of at least three independent experiments *P < 0.05, **P < 0.01.
Mentions: Because SUM1315-bo cells have higher migratory potential than MCF-7 cells, we speculated that miR-106b might be involved in BC migration. To confirm this hypothesis, we transfected SUM1315-bo cells with 200 nM miR-106b mimic to determine whether overexpression of miR-106b would decrease migration and invasion. Using miRNA-specific quantitative real-time PCR, we found that the level of mature miR-106b had increased by 10.55-fold after transfection (Fig.3a). We further analyzed the effects of miR-106b on the migratory and invasive behavior of SUM1315-bo cells. Results showed a 1.83-fold decrease in cell motility and a 5.01-fold decrease in cell invasiveness, respectively, after transfection of the miR-106b mimic (Fig.3b). These results indicate that overexpression of miR-106b can inhibit migration and invasion of SUM1315-bo cells in vitro to a considerable extent.

Bottom Line: MMP2 was shown to be a direct target of miR-106b.Both gain- and loss-of-function studies showed that MMP2 could promote the migration and invasion of BC cells and that miR-106b could suppress both.The blockage of MMP2 by RNA interference mimicked the anti-migration and anti-invasion effects of miR-106b, and introduction of MMP2 antagonized the function of miR-106b.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China; State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.

Show MeSH
Related in: MedlinePlus