Limits...
In vitro and ex vivo evaluation of a multi-epitope heparinase vaccine for various malignancies.

Tang XD, Guo SL, Wang GZ, Li N, Wu YY, Fang DC, Fan YH, Yang SM - Cancer Sci. (2013)

Bottom Line: Previous studies have indicated that heparanase (Hpa) might represent a candidate universal tumor-associated antigen.The results showed that multi-epitope vaccines Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 induced higher Hpa-specific lysis of various cancer cells from different tissues in a HLA-A2-restricted and heparanase-specific manner compared with the single epitope vaccines Hpa525, Hpa277, Hpa405, Hpa16, Hpa8, Hpa310, Hpa315 and Hpa363, both in vitro and ex vivo.Therefore, the present study provides theoretical evidence for the use of heparanase multi-epitope vaccines for clinical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Show MeSH

Related in: MedlinePlus

Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317872&req=5

fig06: Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.

Mentions: Because CTL are known to produce the Th1 cytokine IFN-γ, we quantified peptide-specific T cells by measuring IFN-γ-producing cells with an ELISPOT assay. As shown in Figure6, Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 and their corresponding single peptides generated strong peptide-specific T-cell responses by inducing a higher frequency of IFN-γ-producing T cells compared with a control peptide (P < 0.05). We also found that mice immunized with Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 produced many more IFN-γ spots (indicating peptide-specific T cells) than mice immunized with vaccines expressing the corresponding single peptides (P < 0.05) (Fig.6).


In vitro and ex vivo evaluation of a multi-epitope heparinase vaccine for various malignancies.

Tang XD, Guo SL, Wang GZ, Li N, Wu YY, Fang DC, Fan YH, Yang SM - Cancer Sci. (2013)

Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317872&req=5

fig06: Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.
Mentions: Because CTL are known to produce the Th1 cytokine IFN-γ, we quantified peptide-specific T cells by measuring IFN-γ-producing cells with an ELISPOT assay. As shown in Figure6, Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 and their corresponding single peptides generated strong peptide-specific T-cell responses by inducing a higher frequency of IFN-γ-producing T cells compared with a control peptide (P < 0.05). We also found that mice immunized with Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 produced many more IFN-γ spots (indicating peptide-specific T cells) than mice immunized with vaccines expressing the corresponding single peptides (P < 0.05) (Fig.6).

Bottom Line: Previous studies have indicated that heparanase (Hpa) might represent a candidate universal tumor-associated antigen.The results showed that multi-epitope vaccines Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 induced higher Hpa-specific lysis of various cancer cells from different tissues in a HLA-A2-restricted and heparanase-specific manner compared with the single epitope vaccines Hpa525, Hpa277, Hpa405, Hpa16, Hpa8, Hpa310, Hpa315 and Hpa363, both in vitro and ex vivo.Therefore, the present study provides theoretical evidence for the use of heparanase multi-epitope vaccines for clinical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Show MeSH
Related in: MedlinePlus