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In vitro and ex vivo evaluation of a multi-epitope heparinase vaccine for various malignancies.

Tang XD, Guo SL, Wang GZ, Li N, Wu YY, Fang DC, Fan YH, Yang SM - Cancer Sci. (2013)

Bottom Line: The results showed that multi-epitope vaccines Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 induced higher Hpa-specific lysis of various cancer cells from different tissues in a HLA-A2-restricted and heparanase-specific manner compared with the single epitope vaccines Hpa525, Hpa277, Hpa405, Hpa16, Hpa8, Hpa310, Hpa315 and Hpa363, both in vitro and ex vivo.However, these heparanase-specific CTL did not lyse heparanase-expressing autologous lymphocytes and dendritic cells, which confirms the safety of these multi-epitope vaccines.Therefore, the present study provides theoretical evidence for the use of heparanase multi-epitope vaccines for clinical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.

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Related in: MedlinePlus

Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.
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fig06: Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.

Mentions: Because CTL are known to produce the Th1 cytokine IFN-γ, we quantified peptide-specific T cells by measuring IFN-γ-producing cells with an ELISPOT assay. As shown in Figure6, Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 and their corresponding single peptides generated strong peptide-specific T-cell responses by inducing a higher frequency of IFN-γ-producing T cells compared with a control peptide (P < 0.05). We also found that mice immunized with Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 produced many more IFN-γ spots (indicating peptide-specific T cells) than mice immunized with vaccines expressing the corresponding single peptides (P < 0.05) (Fig.6).


In vitro and ex vivo evaluation of a multi-epitope heparinase vaccine for various malignancies.

Tang XD, Guo SL, Wang GZ, Li N, Wu YY, Fang DC, Fan YH, Yang SM - Cancer Sci. (2013)

Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317872&req=5

fig06: Interferon (IFN)-γ-producing cell quantification using an enzyme-linked immunospot (ELISPOT) assay. The negative peptide (NP), HLA-A2-restricted nonapeptide HIVpol(476-484)(ILLEPVHGV) derived from HIV, serves as the NP control. Phytohemagglutinin (PHA) was used as a positive control. The column indicates the mean; bars, SE. *Statistically significant values (P < 0.01 using a paired Student's t test) were assessed compared with the NP group.
Mentions: Because CTL are known to produce the Th1 cytokine IFN-γ, we quantified peptide-specific T cells by measuring IFN-γ-producing cells with an ELISPOT assay. As shown in Figure6, Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 and their corresponding single peptides generated strong peptide-specific T-cell responses by inducing a higher frequency of IFN-γ-producing T cells compared with a control peptide (P < 0.05). We also found that mice immunized with Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 produced many more IFN-γ spots (indicating peptide-specific T cells) than mice immunized with vaccines expressing the corresponding single peptides (P < 0.05) (Fig.6).

Bottom Line: The results showed that multi-epitope vaccines Hpa525 + 277 + 405 + 16 and Hpa8 + 310 + 315 + 363 induced higher Hpa-specific lysis of various cancer cells from different tissues in a HLA-A2-restricted and heparanase-specific manner compared with the single epitope vaccines Hpa525, Hpa277, Hpa405, Hpa16, Hpa8, Hpa310, Hpa315 and Hpa363, both in vitro and ex vivo.However, these heparanase-specific CTL did not lyse heparanase-expressing autologous lymphocytes and dendritic cells, which confirms the safety of these multi-epitope vaccines.Therefore, the present study provides theoretical evidence for the use of heparanase multi-epitope vaccines for clinical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Show MeSH
Related in: MedlinePlus