Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.
Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.Show MeSH
Related in: MedlinePlus
Mentions: As shown in Figure8(a), MSCs cocultured with C6 glioma cells were treated with 0–50 μM STA-21for 24 h and the cell proliferation inhibition rate was analyzed using MTT. Significant cell growth inhibition was observed in STA-21 treatment groups compared with the group without STA-21 treatment (P < 0.05). Analyses using Western immunoblotting (Fig.8b) indicated that the expression of STAT3, p-STAT3, CyclinD1, and Bcl-xl was significantly decreased in STA-21 treatment groups compared to the group without STA-21 treatment (P < 0.05).
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.