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Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

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Related in: MedlinePlus

Effect of inhibition of signal transducer and activator of transcription 3 (STAT3) by STA-21. (a) Mesenchymal stem cells (MSCs) cocultured with C6 glioma cells were treated with 0–50 μM STA-21 for 24 h then the cell proliferation inhibition rate was analyzed using MTT. (b) Western blotting data showed that the protein expression levels of STAT3, p-STAT3, CyclinD1, and Bcl-xl in STA-21-treated groups were significantly increased compared to the group without STA-21 treatment.
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fig08: Effect of inhibition of signal transducer and activator of transcription 3 (STAT3) by STA-21. (a) Mesenchymal stem cells (MSCs) cocultured with C6 glioma cells were treated with 0–50 μM STA-21 for 24 h then the cell proliferation inhibition rate was analyzed using MTT. (b) Western blotting data showed that the protein expression levels of STAT3, p-STAT3, CyclinD1, and Bcl-xl in STA-21-treated groups were significantly increased compared to the group without STA-21 treatment.

Mentions: As shown in Figure8(a), MSCs cocultured with C6 glioma cells were treated with 0–50 μM STA-21for 24 h and the cell proliferation inhibition rate was analyzed using MTT. Significant cell growth inhibition was observed in STA-21 treatment groups compared with the group without STA-21 treatment (P < 0.05). Analyses using Western immunoblotting (Fig.8b) indicated that the expression of STAT3, p-STAT3, CyclinD1, and Bcl-xl was significantly decreased in STA-21 treatment groups compared to the group without STA-21 treatment (P < 0.05).


Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Effect of inhibition of signal transducer and activator of transcription 3 (STAT3) by STA-21. (a) Mesenchymal stem cells (MSCs) cocultured with C6 glioma cells were treated with 0–50 μM STA-21 for 24 h then the cell proliferation inhibition rate was analyzed using MTT. (b) Western blotting data showed that the protein expression levels of STAT3, p-STAT3, CyclinD1, and Bcl-xl in STA-21-treated groups were significantly increased compared to the group without STA-21 treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317870&req=5

fig08: Effect of inhibition of signal transducer and activator of transcription 3 (STAT3) by STA-21. (a) Mesenchymal stem cells (MSCs) cocultured with C6 glioma cells were treated with 0–50 μM STA-21 for 24 h then the cell proliferation inhibition rate was analyzed using MTT. (b) Western blotting data showed that the protein expression levels of STAT3, p-STAT3, CyclinD1, and Bcl-xl in STA-21-treated groups were significantly increased compared to the group without STA-21 treatment.
Mentions: As shown in Figure8(a), MSCs cocultured with C6 glioma cells were treated with 0–50 μM STA-21for 24 h and the cell proliferation inhibition rate was analyzed using MTT. Significant cell growth inhibition was observed in STA-21 treatment groups compared with the group without STA-21 treatment (P < 0.05). Analyses using Western immunoblotting (Fig.8b) indicated that the expression of STAT3, p-STAT3, CyclinD1, and Bcl-xl was significantly decreased in STA-21 treatment groups compared to the group without STA-21 treatment (P < 0.05).

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Show MeSH
Related in: MedlinePlus