Limits...
Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Show MeSH

Related in: MedlinePlus

Injection of mesenchymal stem cells (MSCs) treated with interleukin (IL)-6 generated tumor formation in nude mice. (a) Tumors were observed in nude mice at the site injected with MSCs treated with IL-6 (arrow). (b) H&E staining of tissue from the cell injection site showed clusters of tumor cells with necrosis in the center (arrow). (c) No tumor growth was observed in nude mice after injection of normal control cells. (d) H&E staining of tissues at the cell injection site in the control group showed a normal skeletal muscle structure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317870&req=5

fig07: Injection of mesenchymal stem cells (MSCs) treated with interleukin (IL)-6 generated tumor formation in nude mice. (a) Tumors were observed in nude mice at the site injected with MSCs treated with IL-6 (arrow). (b) H&E staining of tissue from the cell injection site showed clusters of tumor cells with necrosis in the center (arrow). (c) No tumor growth was observed in nude mice after injection of normal control cells. (d) H&E staining of tissues at the cell injection site in the control group showed a normal skeletal muscle structure.

Mentions: When IL-6-treated cells were injected into the skin of nude mice, visible tumor growth was observed in the injection site 3 months after injection (Fig.7a), whereas no tumor growth was present at the site injected with control cells (Fig.7c). Staining with H&E revealed different sized clusters of amorphous tumor cells without specific cell orientation in the injection site with the IL-6-treated cells (Fig.7b). Necrotic cells were visible in the center of some cell clusters. Only normal skeletal muscle structure was seen on H&E staining in the inoculation site with control cells (Fig.7d).


Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Injection of mesenchymal stem cells (MSCs) treated with interleukin (IL)-6 generated tumor formation in nude mice. (a) Tumors were observed in nude mice at the site injected with MSCs treated with IL-6 (arrow). (b) H&E staining of tissue from the cell injection site showed clusters of tumor cells with necrosis in the center (arrow). (c) No tumor growth was observed in nude mice after injection of normal control cells. (d) H&E staining of tissues at the cell injection site in the control group showed a normal skeletal muscle structure.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317870&req=5

fig07: Injection of mesenchymal stem cells (MSCs) treated with interleukin (IL)-6 generated tumor formation in nude mice. (a) Tumors were observed in nude mice at the site injected with MSCs treated with IL-6 (arrow). (b) H&E staining of tissue from the cell injection site showed clusters of tumor cells with necrosis in the center (arrow). (c) No tumor growth was observed in nude mice after injection of normal control cells. (d) H&E staining of tissues at the cell injection site in the control group showed a normal skeletal muscle structure.
Mentions: When IL-6-treated cells were injected into the skin of nude mice, visible tumor growth was observed in the injection site 3 months after injection (Fig.7a), whereas no tumor growth was present at the site injected with control cells (Fig.7c). Staining with H&E revealed different sized clusters of amorphous tumor cells without specific cell orientation in the injection site with the IL-6-treated cells (Fig.7b). Necrotic cells were visible in the center of some cell clusters. Only normal skeletal muscle structure was seen on H&E staining in the inoculation site with control cells (Fig.7d).

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Show MeSH
Related in: MedlinePlus