Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.
Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.Show MeSH
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Mentions: When IL-6-treated cells were injected into the skin of nude mice, visible tumor growth was observed in the injection site 3 months after injection (Fig.7a), whereas no tumor growth was present at the site injected with control cells (Fig.7c). Staining with H&E revealed different sized clusters of amorphous tumor cells without specific cell orientation in the injection site with the IL-6-treated cells (Fig.7b). Necrotic cells were visible in the center of some cell clusters. Only normal skeletal muscle structure was seen on H&E staining in the inoculation site with control cells (Fig.7d).
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.