Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.
Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.Show MeSH
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Mentions: As shown in Figure4, the levels of HGF and IL-6 were significantly increased in the conditioned media of the MSCs cocultured with C6 glioma cells compared to that of normal MSCs or the MSCs cocultured with normal astrocytes (P < 0.01). However, no significant change in the expression of bFGF was observed in the cells cocultured with C6 glioma cells (P > 0.05). There was no significant difference in the expression of HGF, IL-6, or bFGF between normal MSCs and the cells cocultured with astrocytes. Analysis with ELISA showed that the level of sIL-6R in the conditioned media of glioma cells and the cells cocultured with C6 glioma cells was also significantly increased (Fig.5a) along with an increased expression of the membrane protein GP130 (as determined by immunofluorescence staining in these cells as compared with normal MSCs (Fig.5b) (P < 0.05).
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.