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Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

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Levels of hepatocyte growth factor (HGF), interleukin (IL)-6, and basic fibroblast growth factor (bFGF) detected in the conditioned media from mesenchymal stem cells (MSCs) cocultured with C6 glioma cells. Analysis with ELISA showed that expression levels of HGF and IL-6 were increased significantly in the MSC media cocultured with C6 glioma cells, whereas bFGF expression was not significantly changed in MSCs cocultured with tumor cells compared to the control group. No significant difference in the expression of HGF, IL-6, or bFGF was observed in the control group.
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fig04: Levels of hepatocyte growth factor (HGF), interleukin (IL)-6, and basic fibroblast growth factor (bFGF) detected in the conditioned media from mesenchymal stem cells (MSCs) cocultured with C6 glioma cells. Analysis with ELISA showed that expression levels of HGF and IL-6 were increased significantly in the MSC media cocultured with C6 glioma cells, whereas bFGF expression was not significantly changed in MSCs cocultured with tumor cells compared to the control group. No significant difference in the expression of HGF, IL-6, or bFGF was observed in the control group.

Mentions: As shown in Figure4, the levels of HGF and IL-6 were significantly increased in the conditioned media of the MSCs cocultured with C6 glioma cells compared to that of normal MSCs or the MSCs cocultured with normal astrocytes (P < 0.01). However, no significant change in the expression of bFGF was observed in the cells cocultured with C6 glioma cells (P > 0.05). There was no significant difference in the expression of HGF, IL-6, or bFGF between normal MSCs and the cells cocultured with astrocytes. Analysis with ELISA showed that the level of sIL-6R in the conditioned media of glioma cells and the cells cocultured with C6 glioma cells was also significantly increased (Fig.5a) along with an increased expression of the membrane protein GP130 (as determined by immunofluorescence staining in these cells as compared with normal MSCs (Fig.5b) (P < 0.05).


Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Levels of hepatocyte growth factor (HGF), interleukin (IL)-6, and basic fibroblast growth factor (bFGF) detected in the conditioned media from mesenchymal stem cells (MSCs) cocultured with C6 glioma cells. Analysis with ELISA showed that expression levels of HGF and IL-6 were increased significantly in the MSC media cocultured with C6 glioma cells, whereas bFGF expression was not significantly changed in MSCs cocultured with tumor cells compared to the control group. No significant difference in the expression of HGF, IL-6, or bFGF was observed in the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317870&req=5

fig04: Levels of hepatocyte growth factor (HGF), interleukin (IL)-6, and basic fibroblast growth factor (bFGF) detected in the conditioned media from mesenchymal stem cells (MSCs) cocultured with C6 glioma cells. Analysis with ELISA showed that expression levels of HGF and IL-6 were increased significantly in the MSC media cocultured with C6 glioma cells, whereas bFGF expression was not significantly changed in MSCs cocultured with tumor cells compared to the control group. No significant difference in the expression of HGF, IL-6, or bFGF was observed in the control group.
Mentions: As shown in Figure4, the levels of HGF and IL-6 were significantly increased in the conditioned media of the MSCs cocultured with C6 glioma cells compared to that of normal MSCs or the MSCs cocultured with normal astrocytes (P < 0.01). However, no significant change in the expression of bFGF was observed in the cells cocultured with C6 glioma cells (P > 0.05). There was no significant difference in the expression of HGF, IL-6, or bFGF between normal MSCs and the cells cocultured with astrocytes. Analysis with ELISA showed that the level of sIL-6R in the conditioned media of glioma cells and the cells cocultured with C6 glioma cells was also significantly increased (Fig.5a) along with an increased expression of the membrane protein GP130 (as determined by immunofluorescence staining in these cells as compared with normal MSCs (Fig.5b) (P < 0.05).

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Show MeSH
Related in: MedlinePlus