Limits...
Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Show MeSH

Related in: MedlinePlus

Characteristics of mesenchymal stem cells (MSCs) after coculture with C6 glioma cells. (a) Microscopic examination revealed that the cells showed tumor cell-like morphology a few days after indirect coculture with C6 glioma cells. (b) MTT assay showed the cells after indirect coculture with C6 glioma cells had a growth curve identical to that of cultured glioma cells with a much faster cell proliferation rate than that of control cells. *P < 0.05 compared to control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317870&req=5

fig02: Characteristics of mesenchymal stem cells (MSCs) after coculture with C6 glioma cells. (a) Microscopic examination revealed that the cells showed tumor cell-like morphology a few days after indirect coculture with C6 glioma cells. (b) MTT assay showed the cells after indirect coculture with C6 glioma cells had a growth curve identical to that of cultured glioma cells with a much faster cell proliferation rate than that of control cells. *P < 0.05 compared to control cells.

Mentions: As shown in Figure2(a), the morphology of the cells cocultured with C6 glioma cells was significantly changed. The cytoplasm contracted toward the perinuclear area, and the cells became longer and thinner with smaller nuclei compared to normal MSCs, which were similar to the glioma cells. The cells in this group also grew much faster than the normal MSCs. The growth curve of the MSCs cocultured with C6 glioma cells was similar to that of glioma cells, as shown in Figure2(b) (P < 0.05), suggesting malignant transformation of MSCs after coculture with C6 glioma cells.


Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.

Cui X, Liu J, Bai L, Tian J, Zhu J - Cancer Sci. (2013)

Characteristics of mesenchymal stem cells (MSCs) after coculture with C6 glioma cells. (a) Microscopic examination revealed that the cells showed tumor cell-like morphology a few days after indirect coculture with C6 glioma cells. (b) MTT assay showed the cells after indirect coculture with C6 glioma cells had a growth curve identical to that of cultured glioma cells with a much faster cell proliferation rate than that of control cells. *P < 0.05 compared to control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317870&req=5

fig02: Characteristics of mesenchymal stem cells (MSCs) after coculture with C6 glioma cells. (a) Microscopic examination revealed that the cells showed tumor cell-like morphology a few days after indirect coculture with C6 glioma cells. (b) MTT assay showed the cells after indirect coculture with C6 glioma cells had a growth curve identical to that of cultured glioma cells with a much faster cell proliferation rate than that of control cells. *P < 0.05 compared to control cells.
Mentions: As shown in Figure2(a), the morphology of the cells cocultured with C6 glioma cells was significantly changed. The cytoplasm contracted toward the perinuclear area, and the cells became longer and thinner with smaller nuclei compared to normal MSCs, which were similar to the glioma cells. The cells in this group also grew much faster than the normal MSCs. The growth curve of the MSCs cocultured with C6 glioma cells was similar to that of glioma cells, as shown in Figure2(b) (P < 0.05), suggesting malignant transformation of MSCs after coculture with C6 glioma cells.

Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.

Show MeSH
Related in: MedlinePlus