Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.
Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.Show MeSH
Related in: MedlinePlus
Mentions: As shown in Figure2(a), the morphology of the cells cocultured with C6 glioma cells was significantly changed. The cytoplasm contracted toward the perinuclear area, and the cells became longer and thinner with smaller nuclei compared to normal MSCs, which were similar to the glioma cells. The cells in this group also grew much faster than the normal MSCs. The growth curve of the MSCs cocultured with C6 glioma cells was similar to that of glioma cells, as shown in Figure2(b) (P < 0.05), suggesting malignant transformation of MSCs after coculture with C6 glioma cells.
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.