Interleukin-6 induces malignant transformation of rat mesenchymal stem cells in association with enhanced signaling of signal transducer and activator of transcription 3.
Bottom Line: The expression of IL-6 was significantly increased in MSCs cocultured with glioma cells, which was associated with significantly increased expressions of soluble IL-6 receptor, transmembrane glycoprotein GP130, STAT3, phosphorylated STAT3, CyclinD1, and Bcl-xl.Similar results were obtained when the MSCs were treated with IL-6.These data suggest that IL-6 plays a critical role in malignant transformation of rat MSCs, which is associated with an enhancement of the STAT3 signaling pathway in the tumor microenvironment.
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.Show MeSH
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Mentions: To ensure that the cells used in the present study were astrocytes and MSCs, the phenotype of the cells was determined by measuring the specific cell markers for rat astrocyte GFAP and MSC CD71 and CD105, respectively. As shown in Figure1(a), more than 95% of the astrocytes and C6 glioma cells under immunofluorescence staining were positive for GFAP, but the ratio of the expression of MSCs was only 13%, confirming that these cells were astrocytes. As expected, more than 95% of the cells stained double-positive for CD71 and CD105, confirming that these cells were MSCs (Fig.1b,c).
Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.