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Th17 cells and interleukin-17 increase with poor prognosis in patients with acute myeloid leukemia.

Han Y, Ye A, Bi L, Wu J, Yu K, Zhang S - Cancer Sci. (2014)

Bottom Line: Plasma levels of interleukin (IL)-17, IL-22, IL-23, IL-1β, IL-6, and transforming growth factor (TGF)-β1 were significantly increased in blood and bone marrow in AML patients compared with healthy donors.Patients with high Th17 cell frequency had poor prognosis, whereas patients with high Th1 cell frequency had prolonged survival.Combined analysis of Th1 and Th17 cell frequencies improved the ability to predict patient outcomes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

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Phenotype of tumor-infiltrating Th17 cells. After stimulation with phorbol 12-myristate13-acetate (PMA) and ionomycin for 5 h, freshly isolated bone marrow mononuclear cells (BMMCs) were subjected to membrane and intracellular staining and analyzed by flow cytometry. Representative data were shown from 21 untreated AML patients. (a) Interleukin (IL)-17A expression in T and B cells. IL-17A expression was analyzed in BMMCs. (b) The expression of HLA-DR, CD25, CCR6, CD45RA, CD45RO, and CD62L in tumor-infiltrating Th17 cells.
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fig02: Phenotype of tumor-infiltrating Th17 cells. After stimulation with phorbol 12-myristate13-acetate (PMA) and ionomycin for 5 h, freshly isolated bone marrow mononuclear cells (BMMCs) were subjected to membrane and intracellular staining and analyzed by flow cytometry. Representative data were shown from 21 untreated AML patients. (a) Interleukin (IL)-17A expression in T and B cells. IL-17A expression was analyzed in BMMCs. (b) The expression of HLA-DR, CD25, CCR6, CD45RA, CD45RO, and CD62L in tumor-infiltrating Th17 cells.

Mentions: Higher Th17 cell frequencies in AML patients compared with those in healthy donors were shown, which provoked our interests to examine the phenotype of Th17 cells in BM, a tumor microenvironment. As shown in Figure 2(a), we found that IL-17A was mainly produced by T cells rather than B cells. The majority of tumor-infiltrating IL-17A+ T cells were IL-17A+CD4+ (Th17) cells but not IL-17A+CD8+ cells. Tumor-infiltrating Th17 cells express high levels of CCR6 and negligible levels of HLA-DR, CD25, and CD62L (Fig. 2b). CCR6 is a surface receptor of Th17 cells and Th17 cells can be migrated towards tumor in a CCR6/CCL20 dependent manner, which leads to an enrichment of Th17 cells in the tumor microenvironment.(24) We also observed that Tumor-infiltrating Th17 cells were mainly CD4+CD45RO+ memory T cells, but not CD4+CD45RA+ naive T cells.


Th17 cells and interleukin-17 increase with poor prognosis in patients with acute myeloid leukemia.

Han Y, Ye A, Bi L, Wu J, Yu K, Zhang S - Cancer Sci. (2014)

Phenotype of tumor-infiltrating Th17 cells. After stimulation with phorbol 12-myristate13-acetate (PMA) and ionomycin for 5 h, freshly isolated bone marrow mononuclear cells (BMMCs) were subjected to membrane and intracellular staining and analyzed by flow cytometry. Representative data were shown from 21 untreated AML patients. (a) Interleukin (IL)-17A expression in T and B cells. IL-17A expression was analyzed in BMMCs. (b) The expression of HLA-DR, CD25, CCR6, CD45RA, CD45RO, and CD62L in tumor-infiltrating Th17 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317867&req=5

fig02: Phenotype of tumor-infiltrating Th17 cells. After stimulation with phorbol 12-myristate13-acetate (PMA) and ionomycin for 5 h, freshly isolated bone marrow mononuclear cells (BMMCs) were subjected to membrane and intracellular staining and analyzed by flow cytometry. Representative data were shown from 21 untreated AML patients. (a) Interleukin (IL)-17A expression in T and B cells. IL-17A expression was analyzed in BMMCs. (b) The expression of HLA-DR, CD25, CCR6, CD45RA, CD45RO, and CD62L in tumor-infiltrating Th17 cells.
Mentions: Higher Th17 cell frequencies in AML patients compared with those in healthy donors were shown, which provoked our interests to examine the phenotype of Th17 cells in BM, a tumor microenvironment. As shown in Figure 2(a), we found that IL-17A was mainly produced by T cells rather than B cells. The majority of tumor-infiltrating IL-17A+ T cells were IL-17A+CD4+ (Th17) cells but not IL-17A+CD8+ cells. Tumor-infiltrating Th17 cells express high levels of CCR6 and negligible levels of HLA-DR, CD25, and CD62L (Fig. 2b). CCR6 is a surface receptor of Th17 cells and Th17 cells can be migrated towards tumor in a CCR6/CCL20 dependent manner, which leads to an enrichment of Th17 cells in the tumor microenvironment.(24) We also observed that Tumor-infiltrating Th17 cells were mainly CD4+CD45RO+ memory T cells, but not CD4+CD45RA+ naive T cells.

Bottom Line: Plasma levels of interleukin (IL)-17, IL-22, IL-23, IL-1β, IL-6, and transforming growth factor (TGF)-β1 were significantly increased in blood and bone marrow in AML patients compared with healthy donors.Patients with high Th17 cell frequency had poor prognosis, whereas patients with high Th1 cell frequency had prolonged survival.Combined analysis of Th1 and Th17 cell frequencies improved the ability to predict patient outcomes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Show MeSH
Related in: MedlinePlus