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Th17 cells and interleukin-17 increase with poor prognosis in patients with acute myeloid leukemia.

Han Y, Ye A, Bi L, Wu J, Yu K, Zhang S - Cancer Sci. (2014)

Bottom Line: Plasma levels of interleukin (IL)-17, IL-22, IL-23, IL-1β, IL-6, and transforming growth factor (TGF)-β1 were significantly increased in blood and bone marrow in AML patients compared with healthy donors.Patients with high Th17 cell frequency had poor prognosis, whereas patients with high Th1 cell frequency had prolonged survival.Combined analysis of Th1 and Th17 cell frequencies improved the ability to predict patient outcomes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

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Elevated frequencies of Th17 cells and reduced frequencies of Th1 cells in freshly isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) from acute myeloid leukemia (AML) patients. (a) PBMCs and BMMCs were isolated from AML patients and healthy donors (HDs) and stimulated for 5 h with phorbol 12-myristate13-acetate (PMA) and ionomycin in the presence of brefeldin A and then stained for CD3, CD8, intracellular interleukin (IL)-17A and interferon (IFN)-γ. Frequencies of Th17 cells and Th1 cells were determined by flow cytometry. Representative dot plots using matching peripheral blood (PB) and bone marrow (BM) samples from AML patients and HDs were shown. (b) Collective results presented for Th17 and Th1 cells within CD4+ T population. (c) Total RNA was isolated from CD4+ T cells obtained from AML patients and HDs and reverse transcribed into cDNA and subsequently real time polymerase chain reaction (PCR) for IL-17A and IFN-γ. Results were expressed as mean ± SEM.
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fig01: Elevated frequencies of Th17 cells and reduced frequencies of Th1 cells in freshly isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) from acute myeloid leukemia (AML) patients. (a) PBMCs and BMMCs were isolated from AML patients and healthy donors (HDs) and stimulated for 5 h with phorbol 12-myristate13-acetate (PMA) and ionomycin in the presence of brefeldin A and then stained for CD3, CD8, intracellular interleukin (IL)-17A and interferon (IFN)-γ. Frequencies of Th17 cells and Th1 cells were determined by flow cytometry. Representative dot plots using matching peripheral blood (PB) and bone marrow (BM) samples from AML patients and HDs were shown. (b) Collective results presented for Th17 and Th1 cells within CD4+ T population. (c) Total RNA was isolated from CD4+ T cells obtained from AML patients and HDs and reverse transcribed into cDNA and subsequently real time polymerase chain reaction (PCR) for IL-17A and IFN-γ. Results were expressed as mean ± SEM.

Mentions: We evaluated the frequencies of Th17 cells in freshly isolated PBMCs and BMMCs from AML patients and compared them with those in PBMCs and BMMCs from healthy donors. After gating on CD3+CD8- T cells, intracellular production of IL-17A and IFN-γ were analyzed. The frequencies of Th17 cells were 3.59 ± 0.37% in AML PBMCs compared with 1.47 ± 0.14% in healthy donor PBMCs (P < 0.01) and 3.40 ± 0.21% in AML BMMCs compared with 1.51 ± 0.48% in healthy donor BMMCs (P < 0.01) (Fig. 1b). The frequencies of Th17 cells were significantly increased in PBMCs and BMMCs from AML patients compared with those in healthy donor PBMCs and BMMCs, whereas the frequencies of Th1 cells were significantly decreased in AML PBMCs and BMMCs compared to healthy donor PBMCs and BMMCs (Fig. 1a,b). We further confirmed elevated frequencies of IL-17A-producing cells in CD4+ cells from AML patients compared to healthy donors by qPCR, while IFN-γ-producing cells, although high, is not statistically significant by qPCR (Fig. 1c).


Th17 cells and interleukin-17 increase with poor prognosis in patients with acute myeloid leukemia.

Han Y, Ye A, Bi L, Wu J, Yu K, Zhang S - Cancer Sci. (2014)

Elevated frequencies of Th17 cells and reduced frequencies of Th1 cells in freshly isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) from acute myeloid leukemia (AML) patients. (a) PBMCs and BMMCs were isolated from AML patients and healthy donors (HDs) and stimulated for 5 h with phorbol 12-myristate13-acetate (PMA) and ionomycin in the presence of brefeldin A and then stained for CD3, CD8, intracellular interleukin (IL)-17A and interferon (IFN)-γ. Frequencies of Th17 cells and Th1 cells were determined by flow cytometry. Representative dot plots using matching peripheral blood (PB) and bone marrow (BM) samples from AML patients and HDs were shown. (b) Collective results presented for Th17 and Th1 cells within CD4+ T population. (c) Total RNA was isolated from CD4+ T cells obtained from AML patients and HDs and reverse transcribed into cDNA and subsequently real time polymerase chain reaction (PCR) for IL-17A and IFN-γ. Results were expressed as mean ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317867&req=5

fig01: Elevated frequencies of Th17 cells and reduced frequencies of Th1 cells in freshly isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) from acute myeloid leukemia (AML) patients. (a) PBMCs and BMMCs were isolated from AML patients and healthy donors (HDs) and stimulated for 5 h with phorbol 12-myristate13-acetate (PMA) and ionomycin in the presence of brefeldin A and then stained for CD3, CD8, intracellular interleukin (IL)-17A and interferon (IFN)-γ. Frequencies of Th17 cells and Th1 cells were determined by flow cytometry. Representative dot plots using matching peripheral blood (PB) and bone marrow (BM) samples from AML patients and HDs were shown. (b) Collective results presented for Th17 and Th1 cells within CD4+ T population. (c) Total RNA was isolated from CD4+ T cells obtained from AML patients and HDs and reverse transcribed into cDNA and subsequently real time polymerase chain reaction (PCR) for IL-17A and IFN-γ. Results were expressed as mean ± SEM.
Mentions: We evaluated the frequencies of Th17 cells in freshly isolated PBMCs and BMMCs from AML patients and compared them with those in PBMCs and BMMCs from healthy donors. After gating on CD3+CD8- T cells, intracellular production of IL-17A and IFN-γ were analyzed. The frequencies of Th17 cells were 3.59 ± 0.37% in AML PBMCs compared with 1.47 ± 0.14% in healthy donor PBMCs (P < 0.01) and 3.40 ± 0.21% in AML BMMCs compared with 1.51 ± 0.48% in healthy donor BMMCs (P < 0.01) (Fig. 1b). The frequencies of Th17 cells were significantly increased in PBMCs and BMMCs from AML patients compared with those in healthy donor PBMCs and BMMCs, whereas the frequencies of Th1 cells were significantly decreased in AML PBMCs and BMMCs compared to healthy donor PBMCs and BMMCs (Fig. 1a,b). We further confirmed elevated frequencies of IL-17A-producing cells in CD4+ cells from AML patients compared to healthy donors by qPCR, while IFN-γ-producing cells, although high, is not statistically significant by qPCR (Fig. 1c).

Bottom Line: Plasma levels of interleukin (IL)-17, IL-22, IL-23, IL-1β, IL-6, and transforming growth factor (TGF)-β1 were significantly increased in blood and bone marrow in AML patients compared with healthy donors.Patients with high Th17 cell frequency had poor prognosis, whereas patients with high Th1 cell frequency had prolonged survival.Combined analysis of Th1 and Th17 cell frequencies improved the ability to predict patient outcomes.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Show MeSH
Related in: MedlinePlus