Limits...
Transforming growth factor-β-induced lncRNA-Smad7 inhibits apoptosis of mouse breast cancer JygMC(A) cells.

Arase M, Horiguchi K, Ehata S, Morikawa M, Tsutsumi S, Aburatani H, Miyazono K, Koinuma D - Cancer Sci. (2014)

Bottom Line: Transforming growth factor (TGF)-β exhibits both pro-apoptotic and anti-apoptotic effects on epithelial cells in a context-dependent manner.The anti-apoptotic effect of lncRNA-Smad7 appeared to occur independently of the transcriptional regulation by TGF-β of anti-apoptotic DEC1 and pro-apoptotic Bim proteins.Small interfering RNA for lncRNA-Smad7 did not alter the process of TGF-β-induced epithelial-mesenchymal transition, phosphorylation of Smad2 or expression of the Smad7 gene, suggesting that the contribution of this lncRNA to TGF-β functions may be restricted to apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Show MeSH

Related in: MedlinePlus

Knockdown of lncRNA-Smad7 represses tumor-forming ability of JygMc(A) cells. (a) Effect of siRNA for lncRNA-Smad7 (silncRNA-1) on the size of xenografted tumors (n = 6). Photos were taken 8 d after tumor inoculation. Representative images are shown in this figure. (b) Sequential tumor volume measurement after inoculation. Error bars: standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4317863&req=5

fig06: Knockdown of lncRNA-Smad7 represses tumor-forming ability of JygMc(A) cells. (a) Effect of siRNA for lncRNA-Smad7 (silncRNA-1) on the size of xenografted tumors (n = 6). Photos were taken 8 d after tumor inoculation. Representative images are shown in this figure. (b) Sequential tumor volume measurement after inoculation. Error bars: standard deviations.

Mentions: Finally, we examined whether lncRNA-Smad7 affects the tumor-forming ability of JygMC(A) cells. Tumor size was periodically measured after subcutaneous inoculation of siRNA-transfected JygMC(A) cells into mice. As shown in Figure 6, knockdown of lncRNA-Smad7 resulted in reduced tumor size, suggesting impaired graft survival in the absence of lncRNA-Smad7 (Fig. 6a,b).


Transforming growth factor-β-induced lncRNA-Smad7 inhibits apoptosis of mouse breast cancer JygMC(A) cells.

Arase M, Horiguchi K, Ehata S, Morikawa M, Tsutsumi S, Aburatani H, Miyazono K, Koinuma D - Cancer Sci. (2014)

Knockdown of lncRNA-Smad7 represses tumor-forming ability of JygMc(A) cells. (a) Effect of siRNA for lncRNA-Smad7 (silncRNA-1) on the size of xenografted tumors (n = 6). Photos were taken 8 d after tumor inoculation. Representative images are shown in this figure. (b) Sequential tumor volume measurement after inoculation. Error bars: standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317863&req=5

fig06: Knockdown of lncRNA-Smad7 represses tumor-forming ability of JygMc(A) cells. (a) Effect of siRNA for lncRNA-Smad7 (silncRNA-1) on the size of xenografted tumors (n = 6). Photos were taken 8 d after tumor inoculation. Representative images are shown in this figure. (b) Sequential tumor volume measurement after inoculation. Error bars: standard deviations.
Mentions: Finally, we examined whether lncRNA-Smad7 affects the tumor-forming ability of JygMC(A) cells. Tumor size was periodically measured after subcutaneous inoculation of siRNA-transfected JygMC(A) cells into mice. As shown in Figure 6, knockdown of lncRNA-Smad7 resulted in reduced tumor size, suggesting impaired graft survival in the absence of lncRNA-Smad7 (Fig. 6a,b).

Bottom Line: Transforming growth factor (TGF)-β exhibits both pro-apoptotic and anti-apoptotic effects on epithelial cells in a context-dependent manner.The anti-apoptotic effect of lncRNA-Smad7 appeared to occur independently of the transcriptional regulation by TGF-β of anti-apoptotic DEC1 and pro-apoptotic Bim proteins.Small interfering RNA for lncRNA-Smad7 did not alter the process of TGF-β-induced epithelial-mesenchymal transition, phosphorylation of Smad2 or expression of the Smad7 gene, suggesting that the contribution of this lncRNA to TGF-β functions may be restricted to apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus