MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer.
Bottom Line: In this study, we found that miR-135a-5p expression is often dampened and correlated with neoplasm histologic grade in GBC.Furthermore, the p38 MAPK pathway was proven to be involved in miR-135a-VLDLR downstream signaling.Together, these results suggested that the miR-135a-VLDLR-p38 axis may contribute to GBC cell proliferation.
Affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.Show MeSH
Related in: MedlinePlus
Mentions: To further address the critical role of VLDLR in miR-135a-5p-induced suppression of GBC cell proliferation, we constructed a lentiviral expression vector of VLDLR ORF without the 3′-UTR, and established the stable GBC cells using this vector. The infection of lentivirus carrying VLDLR-ORF antagonized the inhibition of miR-135a-5p on GBC cells proliferation (Fig 4a,b), with VLDLR protein recovery in Lenti-miR-135a cells (Fig 4c). Furthermore, it was shown that enforced expression of VLDLR could counteract the miR-135a-5p-induced G1/S arrest (Fig. S3a). These results suggested that VLDLR is a functional target of miR-135a-5p in GBC cells.
Affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.