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MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer.

Zhou H, Guo W, Zhao Y, Wang Y, Zha R, Ding J, Liang L, Yang G, Chen Z, Ma B, Yin B - Cancer Sci. (2014)

Bottom Line: Moreover, very low density lipoprotein receptor (VLDLR), which is often upregulated in GBC tissues, was identified as a direct functional target of miR-135a-5p.Furthermore, the p38 MAPK pathway was proven to be involved in miR-135a-VLDLR downstream signaling.Together, these results suggested that the miR-135a-VLDLR-p38 axis may contribute to GBC cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.

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Functional screen of microRNAs (miRNAs); miR-135a-5p is often downregulated in gallbladder cancer (GBC). (a) Heatmap of miRNA microarrays resulted in four pairs of GBC tissues and paracancerous tissues. (b) Functional screen of miRNAs in which the expression level changed above 10-fold in chip results. *P < 0.05 (miRNA vs negative control). (c) miR-135a-5p expression level in GBC tissues and paracancerous tissues. (d) miR-135a-3p expression level in GBC tissues and paracancerous tissues.
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fig01: Functional screen of microRNAs (miRNAs); miR-135a-5p is often downregulated in gallbladder cancer (GBC). (a) Heatmap of miRNA microarrays resulted in four pairs of GBC tissues and paracancerous tissues. (b) Functional screen of miRNAs in which the expression level changed above 10-fold in chip results. *P < 0.05 (miRNA vs negative control). (c) miR-135a-5p expression level in GBC tissues and paracancerous tissues. (d) miR-135a-3p expression level in GBC tissues and paracancerous tissues.

Mentions: We identified 23 downregulated miRNAs based on the miRNA chip results of four paired GBC and paracancerous tissues (fold-change >10; Fig. 1a, Table 1) and investigate the roles of these 23 miRNAs on GBC cell proliferation using the CCK-8 assay according to the procedure (Fig. S1). Among them, miR-135a-5p and miR-26a were considered to significantly affect GBC cell proliferation (fold-change <0.8/fold-change >1.2, P < 0.05; Fig 1b). By real-time PCR, we found that miR-135a-5p was significantly downregulated in GBC tissues (Fig. 1c), whereas the expression of miR-135a-3p had no significant difference between GBC tissues and their paracancerous tissues (Figs 1d, S2a). In this study, we used miR-135a-5p for further investigations.


MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer.

Zhou H, Guo W, Zhao Y, Wang Y, Zha R, Ding J, Liang L, Yang G, Chen Z, Ma B, Yin B - Cancer Sci. (2014)

Functional screen of microRNAs (miRNAs); miR-135a-5p is often downregulated in gallbladder cancer (GBC). (a) Heatmap of miRNA microarrays resulted in four pairs of GBC tissues and paracancerous tissues. (b) Functional screen of miRNAs in which the expression level changed above 10-fold in chip results. *P < 0.05 (miRNA vs negative control). (c) miR-135a-5p expression level in GBC tissues and paracancerous tissues. (d) miR-135a-3p expression level in GBC tissues and paracancerous tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317855&req=5

fig01: Functional screen of microRNAs (miRNAs); miR-135a-5p is often downregulated in gallbladder cancer (GBC). (a) Heatmap of miRNA microarrays resulted in four pairs of GBC tissues and paracancerous tissues. (b) Functional screen of miRNAs in which the expression level changed above 10-fold in chip results. *P < 0.05 (miRNA vs negative control). (c) miR-135a-5p expression level in GBC tissues and paracancerous tissues. (d) miR-135a-3p expression level in GBC tissues and paracancerous tissues.
Mentions: We identified 23 downregulated miRNAs based on the miRNA chip results of four paired GBC and paracancerous tissues (fold-change >10; Fig. 1a, Table 1) and investigate the roles of these 23 miRNAs on GBC cell proliferation using the CCK-8 assay according to the procedure (Fig. S1). Among them, miR-135a-5p and miR-26a were considered to significantly affect GBC cell proliferation (fold-change <0.8/fold-change >1.2, P < 0.05; Fig 1b). By real-time PCR, we found that miR-135a-5p was significantly downregulated in GBC tissues (Fig. 1c), whereas the expression of miR-135a-3p had no significant difference between GBC tissues and their paracancerous tissues (Figs 1d, S2a). In this study, we used miR-135a-5p for further investigations.

Bottom Line: Moreover, very low density lipoprotein receptor (VLDLR), which is often upregulated in GBC tissues, was identified as a direct functional target of miR-135a-5p.Furthermore, the p38 MAPK pathway was proven to be involved in miR-135a-VLDLR downstream signaling.Together, these results suggested that the miR-135a-VLDLR-p38 axis may contribute to GBC cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus