MicroRNA-135a acts as a putative tumor suppressor by directly targeting very low density lipoprotein receptor in human gallbladder cancer.
Bottom Line: Moreover, very low density lipoprotein receptor (VLDLR), which is often upregulated in GBC tissues, was identified as a direct functional target of miR-135a-5p.Furthermore, the p38 MAPK pathway was proven to be involved in miR-135a-VLDLR downstream signaling.Together, these results suggested that the miR-135a-VLDLR-p38 axis may contribute to GBC cell proliferation.
Affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: We identified 23 downregulated miRNAs based on the miRNA chip results of four paired GBC and paracancerous tissues (fold-change >10; Fig. 1a, Table 1) and investigate the roles of these 23 miRNAs on GBC cell proliferation using the CCK-8 assay according to the procedure (Fig. S1). Among them, miR-135a-5p and miR-26a were considered to significantly affect GBC cell proliferation (fold-change <0.8/fold-change >1.2, P < 0.05; Fig 1b). By real-time PCR, we found that miR-135a-5p was significantly downregulated in GBC tissues (Fig. 1c), whereas the expression of miR-135a-3p had no significant difference between GBC tissues and their paracancerous tissues (Figs 1d, S2a). In this study, we used miR-135a-5p for further investigations.
Affiliation: Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.