Newly defined aberrant crypt foci as a marker for dysplasia in the rat colon.
Bottom Line: Specifically, decoloring of the azoxymethane-treated rat colon after scoring classical ACF (cACF) resulted in visualization of a subset of aberrant crypts that remained densely stained.Accordingly, we designated those foci harboring either of the two crypt subtypes as dysplasia-associated ACF (dACF).Consequently, integrative scoring of cACF and dACF enabled capture of all early lesions of the colon.
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.Show MeSH
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Mentions: An ideal marker should be simply detected and easily diagnosed, let alone manifest high specificity and sensitivity. In terms of a marker for dysplasia, however, none of the known preneoplastic lesions of the colon meet all these criteria. As an extension of our previous study, we developed a more straightforward method that enables more specific and sensitive detection of dysplasia in this study. Indeed, 94% of the dACF showed dysplasia, while 90% of the dysplastic lesions proved to be dACF, obviously overwhelming cACF as a marker. Inter-relationships between dACF and various other lesions are summarized in Figure 6.
Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.