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Newly defined aberrant crypt foci as a marker for dysplasia in the rat colon.

Ochiai M, Hippo Y, Izumiya M, Watanabe M, Nakagama H - Cancer Sci. (2014)

Bottom Line: Specifically, decoloring of the azoxymethane-treated rat colon after scoring classical ACF (cACF) resulted in visualization of a subset of aberrant crypts that remained densely stained.Accordingly, we designated those foci harboring either of the two crypt subtypes as dysplasia-associated ACF (dACF).Consequently, integrative scoring of cACF and dACF enabled capture of all early lesions of the colon.

View Article: PubMed Central - PubMed

Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.

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Related in: MedlinePlus

Dysplasia-associated aberrant crypt foci (dACF) among other preneoplastic lesions of the colon. A summary of the interrelationship between dACF and other preneoplastic lesions, both on unsectioned colon and in thin sections. The spectra of various lesions along multistep colon carcinogenesis clearly indicate that dACF successfully filled in the gap between mucin-depleted foci (MDF)/flat ACF (fACF) and classical ACF (cACF) in detection of dysplasia. c-type, enlarged crypt and broad opening; cd-type, enlarged crypt and narrow opening; d-type, small crypt and narrow opening.
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fig06: Dysplasia-associated aberrant crypt foci (dACF) among other preneoplastic lesions of the colon. A summary of the interrelationship between dACF and other preneoplastic lesions, both on unsectioned colon and in thin sections. The spectra of various lesions along multistep colon carcinogenesis clearly indicate that dACF successfully filled in the gap between mucin-depleted foci (MDF)/flat ACF (fACF) and classical ACF (cACF) in detection of dysplasia. c-type, enlarged crypt and broad opening; cd-type, enlarged crypt and narrow opening; d-type, small crypt and narrow opening.

Mentions: An ideal marker should be simply detected and easily diagnosed, let alone manifest high specificity and sensitivity. In terms of a marker for dysplasia, however, none of the known preneoplastic lesions of the colon meet all these criteria. As an extension of our previous study, we developed a more straightforward method that enables more specific and sensitive detection of dysplasia in this study. Indeed, 94% of the dACF showed dysplasia, while 90% of the dysplastic lesions proved to be dACF, obviously overwhelming cACF as a marker. Inter-relationships between dACF and various other lesions are summarized in Figure 6.


Newly defined aberrant crypt foci as a marker for dysplasia in the rat colon.

Ochiai M, Hippo Y, Izumiya M, Watanabe M, Nakagama H - Cancer Sci. (2014)

Dysplasia-associated aberrant crypt foci (dACF) among other preneoplastic lesions of the colon. A summary of the interrelationship between dACF and other preneoplastic lesions, both on unsectioned colon and in thin sections. The spectra of various lesions along multistep colon carcinogenesis clearly indicate that dACF successfully filled in the gap between mucin-depleted foci (MDF)/flat ACF (fACF) and classical ACF (cACF) in detection of dysplasia. c-type, enlarged crypt and broad opening; cd-type, enlarged crypt and narrow opening; d-type, small crypt and narrow opening.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4317850&req=5

fig06: Dysplasia-associated aberrant crypt foci (dACF) among other preneoplastic lesions of the colon. A summary of the interrelationship between dACF and other preneoplastic lesions, both on unsectioned colon and in thin sections. The spectra of various lesions along multistep colon carcinogenesis clearly indicate that dACF successfully filled in the gap between mucin-depleted foci (MDF)/flat ACF (fACF) and classical ACF (cACF) in detection of dysplasia. c-type, enlarged crypt and broad opening; cd-type, enlarged crypt and narrow opening; d-type, small crypt and narrow opening.
Mentions: An ideal marker should be simply detected and easily diagnosed, let alone manifest high specificity and sensitivity. In terms of a marker for dysplasia, however, none of the known preneoplastic lesions of the colon meet all these criteria. As an extension of our previous study, we developed a more straightforward method that enables more specific and sensitive detection of dysplasia in this study. Indeed, 94% of the dACF showed dysplasia, while 90% of the dysplastic lesions proved to be dACF, obviously overwhelming cACF as a marker. Inter-relationships between dACF and various other lesions are summarized in Figure 6.

Bottom Line: Specifically, decoloring of the azoxymethane-treated rat colon after scoring classical ACF (cACF) resulted in visualization of a subset of aberrant crypts that remained densely stained.Accordingly, we designated those foci harboring either of the two crypt subtypes as dysplasia-associated ACF (dACF).Consequently, integrative scoring of cACF and dACF enabled capture of all early lesions of the colon.

View Article: PubMed Central - PubMed

Affiliation: Division of Cancer Development System, National Cancer Center Research Institute, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus